Liu D, Lightowlers M W, Rickard M D
University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.
Parasite Immunol. 1992 May;14(3):239-48. doi: 10.1111/j.1365-3024.1992.tb00465.x.
Antibody responses in mice with up to 64 weeks of secondary Echinococcus granulosus hydatidosis were examined by ELISA using hydatid protoscolex antigen (Px), Antigen 5 (Ag5) and Antigen B (AgB), and by immunoelectrophoresis (IEP) using sheep hydatid cyst fluid (SHCF). Anti-Px IgG antibodies, evident from 3-5 days post infection (p.i.), increased steadily until 16 weeks and maintained a high level afterwards. Anti-Ag5 IgG antibodies were negligible up to two weeks, but they showed a small increase around 2-3 weeks which was followed by a big increase around 16 weeks p.i. The high level of anti-Ag5 IgG antibodies persisted to the end of experiment. The level of anti-AgB IgG antibodies remained relatively low throughout infection. Anti-Px IgM antibodies appeared in the early period of infection, but became insignificant as the infection proceeded. Specific IgM antibodies to Ag5 and AgB showed two waves of increase, one between 3 days to 4 weeks p.i. and the other between 16 weeks to 46 weeks p.i. The level of IgA antibodies to Ag5 and AgB was low and only a moderate amount of anti-Px IgA antibodies was detected. Generally, a higher level of serum antibodies are associated with a larger number of mature cysts. Serum samples from 5 of 8 mice harbouring hydatid cysts formed 1-3 bands with SHCF in IEP, including Arc 5, but a precipitation are with AgB was not observed. Analysis of hydatid cyst fluid from the infected mice (MHCF) in IEP also failed to demonstrate AgB. Despite the high levels of antihydatid antibodies generated in the infected mice, protoscoleces appeared to be unhindered in their growth to mature cysts.
采用包虫原头节抗原（Px）、抗原5（Ag5）和抗原B（AgB），通过酶联免疫吸附测定（ELISA）对长达64周的继发性细粒棘球绦虫包虫病小鼠的抗体反应进行检测；采用绵羊包虫囊液（SHCF），通过免疫电泳（IEP）进行检测。抗Px IgG抗体在感染后3 - 5天即可检测到，至16周时稳步增加，此后维持在较高水平。抗Ag5 IgG抗体在两周内可忽略不计，但在感染后2 - 3周左右略有增加，随后在感染后16周左右大幅增加。抗Ag5 IgG抗体的高水平一直持续到实验结束。抗AgB IgG抗体水平在整个感染过程中相对较低。抗Px IgM抗体在感染早期出现，但随着感染进展变得不明显。针对Ag5和AgB的特异性IgM抗体出现两波增加，一波在感染后3天至4周之间，另一波在感染后16周至46周之间。针对Ag5和AgB的IgA抗体水平较低，仅检测到适量的抗Px IgA抗体。一般来说，血清抗体水平越高，成熟囊肿数量越多。8只携带包虫囊肿的小鼠中有5只的血清样本在IEP中与SHCF形成1 - 3条带，包括Arc 5，但未观察到与AgB的沉淀带。对感染小鼠的包虫囊液（MHCF）进行IEP分析也未能显示AgB。尽管感染小鼠产生了高水平的抗包虫抗体，但原头节似乎在生长为成熟囊肿的过程中未受阻碍。
