一种生产针对细粒棘球绦虫抗原5和抗原B的单克隆抗体的策略。

A strategy for production of monoclonal antibodies to Echinococcus granulosus antigen 5 and antigen B.

作者信息

Liu D, Rickard M D, Lightowlers M W

机构信息

University of Melbourne, Veterinary Clinical Centre, Werribee, Victoria, Australia.

出版信息

Int J Parasitol. 1992 Nov;22(7):1013-6. doi: 10.1016/0020-7519(92)90062-p.

DOI:10.1016/0020-7519(92)90062-p

PMID:1459778

Abstract

Serum antibody responses to sheep hydatid cyst fluid (SHCF) and a purified Antigen 5 (Ag5) were examined in ELISA, immunoelectrophoresis (IEP) and immunoprecipitation (IP) to facilitate production of monoclonal antibodies (MAb) to E. granulosus Ag5 and Antigen B (AgB). Although sera from mice immunized with SHCF contained antibodies of various classes, the fusions using these donor mice resulted in mainly anti-AgB MAb, possibly due to the preferential selection of MAb to AgB by the SHCF-based ELISA screening system. Donor mice immunized with Ag5 also produced several classes of antibodies, and the resultant fusions enabled selection of IgG MAb to Ag5.

摘要

在酶联免疫吸附测定（ELISA）、免疫电泳（IEP）和免疫沉淀（IP）中检测了针对绵羊包虫囊液（SHCF）和纯化抗原5（Ag5）的血清抗体反应，以促进针对细粒棘球绦虫Ag5和抗原B（AgB）的单克隆抗体（MAb）的产生。尽管用SHCF免疫的小鼠血清中含有各类抗体，但使用这些供体小鼠进行细胞融合主要产生抗AgB单克隆抗体，这可能是由于基于SHCF的ELISA筛选系统优先选择了针对AgB的单克隆抗体。用Ag5免疫的供体小鼠也产生了几类抗体，并且由此产生的细胞融合能够筛选出针对Ag5的IgG单克隆抗体。