Hedberg Elizabeth L, Kroese-Deutman Henriette C, Shih Charles K, Lemoine Jeremy J, Liebschner Michael A K, Miller Michael J, Yasko Alan W, Crowther Roger S, Carney Darrell H, Mikos Antonios G, Jansen John A
Department of Bioengineering, Rice University, Houston, Texas, USA.
Tissue Eng. 2005 Sep-Oct;11(9-10):1356-67. doi: 10.1089/ten.2005.11.1356.
This study focused on the assessment of radiography, microcomputed tomography, and histology for the evaluation of bone formation in a 15.0-mm defect in the rabbit radius after the implantation of a tissue-engineered construct. Radiography was found to be useful as a noninvasive method for obtaining images of calcified tissue throughout the time course of the experiment. With this method, however, image quality was low, making it difficult to obtain precise information about the location and quantity of the bone formed. Microcomputed tomography was used to create three-dimensional reconstructions of the bone (25-microm resolution). These reconstructions allowed for greater spatial resolution than the radiography, but did not allow for imaging of the implanted scaffold material or the surrounding, nonmineralized tissue. To visualize all materials within the defect area at the cellular level, histology was used. Histological analysis, however, is a destructive technique that did not allow for any further analysis of the samples. Each technique examined here has its own advantages and limitations, but each yields unique information regarding bone regeneration. It is only through the use of all three techniques that complete characterization of the bone growth and tissue/construct responses after implantation in vivo.
本研究聚焦于评估X线摄影、微型计算机断层扫描和组织学，以评价在兔桡骨植入组织工程构建体后15.0毫米缺损处的骨形成情况。研究发现，X线摄影作为一种非侵入性方法，在整个实验过程中可用于获取钙化组织的图像。然而，通过这种方法，图像质量较低，难以获取有关形成骨的位置和数量的精确信息。微型计算机断层扫描用于创建骨的三维重建图像（分辨率为25微米）。这些重建图像比X线摄影具有更高的空间分辨率，但无法对植入的支架材料或周围未矿化组织进行成像。为了在细胞水平上可视化缺损区域内的所有材料，采用了组织学方法。然而，组织学分析是一种破坏性技术，无法对样本进行进一步分析。这里所研究的每种技术都有其自身的优点和局限性，但每种技术都能提供有关骨再生的独特信息。只有通过使用这三种技术，才能全面表征体内植入后骨生长以及组织/构建体的反应。
