超薄切片上RNA的特异性检测。

Specific detection of RNA on ultra-thin sections.

作者信息

Calabuig Juana, de Juan Joaquin, Iborra Francisco J

机构信息

INSTRACAN, Institute of Cancer Research, Juan Chaumel 16, Villena 03400, Alicante, Spain.

出版信息

J Struct Biol. 2005 Nov;152(2):146-8. doi: 10.1016/j.jsb.2005.06.005. Epub 2005 Aug 9.

DOI:10.1016/j.jsb.2005.06.005

PMID:16260152

Abstract

In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material.

摘要

在本文中，我们描述了一种用于RNA超微结构检测的特定方法。我们的方法基于RNA中尿苷残基的“原位”溴化，这使得能够用针对溴脱氧尿苷的特异性抗体检测溴化RNA。通过这种方法，我们可以实现高特异性和高分辨率，并且它可应用于环氧树脂或丙烯酸树脂包埋的材料。