Pignot-Paintrand I, Bressac C
Laboratory of Biology of Reproduction and Development, University of Paris-South Central Hospital of Bicêtre, Le Kremlin Bicetre, France.
Biotech Histochem. 1992 Jan;67(1):55-7. doi: 10.3109/10520299209110007.
Rapid three-dimensional reconstruction of serial sections at the light microscopic and ultrastructural levels was accomplished using a two-step technique. Fixed specimens were embedded in Epon and 1 microns sections were cut and placed on glass slides. One of every four sections was drawn onto transparency film for rapid three-dimensional reconstruction. The semi-thin sections were re-embedded in Epon and sectioned at 90 nm for examination in the electron microscopy.
采用两步技术在光学显微镜和超微结构水平上对连续切片进行快速三维重建。将固定好的标本包埋在环氧树脂中，切成1微米厚的切片并置于载玻片上。每四张切片中的一张被绘制到透明胶片上用于快速三维重建。将半薄切片重新包埋在环氧树脂中，并切成90纳米厚用于电子显微镜检查。
