Lee Eun Ju, Kim Jin, Lee Seoung Ae, Kim Eun Jung, Chun Yong Chan, Ryu Mi Heon, Yook Jong In
Department of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Project for Medical Science Yonsei University, School of Dentistry, Seoul 120-752, Korea.
Exp Mol Med. 2005 Oct 31;37(5):379-90. doi: 10.1038/emm.2005.48.
Since genetic abnormalities of human cancer are greatly geographically dependent, cultural and environmental backgrounds are thought to be closely related to the carcinogenic process. In the present study, eight human cell lines were established by culture from untreated carcinomas of the oral cancer, of which five were from primary oral squamous cell carcinomas (OSC), one from a mucoepidermoid carcinoma (MEC) and one each originating from metastatic OSC and MEC. All the studied tumor lines grew as monolayers, and showed: i) an epithelial origin by the presence of cytokeratin, and ii) tumorigenic potential in nude mice. Western blot analysis revealed i) over expression of EGFR in six of the cell lines ii) decreased expression of E-cadherin in six cell lines compared to normal human oral mucosa. A mutational analysis showed: point mutations of p53 at exon 7, with transversion, and at exon 8, with transition. These well-characterized human YD cell lines should serve as useful tools in the study of the molecular pathogenesis and biological characteristics of head and neck cancer cells, and in the future testing of new therapeutic reagents for oral cancer.
由于人类癌症的基因异常在很大程度上依赖于地理因素，因此文化和环境背景被认为与致癌过程密切相关。在本研究中，通过培养从未经治疗的口腔癌中建立了8种人类细胞系，其中5种来自原发性口腔鳞状细胞癌（OSC），1种来自黏液表皮样癌（MEC），另外1种分别源自转移性OSC和MEC。所有研究的肿瘤细胞系均以单层形式生长，并表现出：i）通过细胞角蛋白的存在表明具有上皮起源，以及ii）在裸鼠中具有致瘤潜力。蛋白质免疫印迹分析显示：i）6种细胞系中表皮生长因子受体（EGFR）过表达，ii）与正常人口腔黏膜相比，6种细胞系中E-钙黏蛋白表达降低。突变分析显示：p53基因第7外显子发生颠换突变，第8外显子发生转换突变。这些特征明确的人类YD细胞系应可作为研究头颈部癌细胞分子发病机制和生物学特性以及未来测试口腔癌新治疗试剂的有用工具。
