Hu Z Y, Sun G Y, Rhodes P G
Department of Child Health, University of Missouri, Columbia.
Alcohol Clin Exp Res. 1992 Jun;16(3):432-5. doi: 10.1111/j.1530-0277.1992.tb01395.x.
Phosphatidylserine is enriched in the brain and has been implicated to play a role in regulating neuronal membrane functions. In this study, three experimental protocols were used to examine the effects of in utero ethanol exposure on phosphatidylserine biosynthesis in rat pup brain, namely, (1) assay of the serine base-exchange enzyme activity in brain microsomes, (2) incubation of brain slices with [3H] serine, and (3) incorporation in vivo of [3H]serine into phosphatidylserine as well as serine-related phospholipids in brain. Results from all three protocols point to a decrease in phosphatidylserine biosynthesis in newborn rat pup cerebrum on exposure to ethanol in utero compared with the pair-fed controls. When in utero ethanol-exposed pups were nursed by mothers given a chow diet, the differences gradually returned to control levels by 17 days of age. The decrease in phosphatidylserine biosynthesis may be important in explaining some of the neuronal deficits associated with in utero ethanol exposure.
磷脂酰丝氨酸在大脑中含量丰富，并且被认为在调节神经元膜功能中发挥作用。在本研究中，采用了三种实验方案来检测子宫内乙醇暴露对幼鼠大脑中磷脂酰丝氨酸生物合成的影响，即：（1）检测脑微粒体中丝氨酸碱基交换酶的活性；（2）用[³H]丝氨酸孵育脑切片；（3）在体内将[³H]丝氨酸掺入脑内的磷脂酰丝氨酸以及丝氨酸相关磷脂中。与配对喂养的对照组相比，所有这三种方案的结果均表明，新生幼鼠大脑在子宫内暴露于乙醇后，磷脂酰丝氨酸的生物合成减少。当子宫内暴露于乙醇的幼鼠由给予普通饮食的母鼠哺育时，到17日龄时，差异逐渐恢复到对照水平。磷脂酰丝氨酸生物合成的减少可能对解释一些与子宫内乙醇暴露相关的神经元缺陷具有重要意义。
