Holmgren N, Valberg S
Experimental Station, Veterinary Institute, Skara, Sweden.
Am J Vet Res. 1992 Jun;53(6):957-60.
Quantitative immunodiffusion in one dimension was performed in 6-mm Duran tubes containing a 1% Nobel agar solution and various dilutions of antisera. A series of dilutions of pure myoglobin in equine sera as well as plasma from horses with rhabdomyolysis were tested. Standard curves were prepared of the migration distance of the formed precipitate from the meniscus of the gel after 3, 6, 12, and 24 hours. The clearest line of precipitate was formed with a 1:20 dilution of antisera in agar. Standard curves were nonlinear and plasma myoglobin could be detected at 2 micrograms of myoglobin/ml or greater. The test was optimal, with an error of 5.6%, when read at 24 hours at approximately 25 C. Tubes with agar could be stored for 6 months at 4 C without affecting the accuracy of the test. The specificity of myoglobin for skeletal or cardiac muscle, and its rapid clearance from serum after muscle necrosis, make it ideally suited for evaluating acute muscle damage and for testing the susceptibility of horses for rhabdomyolysis following an exercise test.
在含有1%诺贝尔琼脂溶液和不同稀释度抗血清的6毫米杜兰管中进行一维定量免疫扩散。对马血清中纯肌红蛋白的一系列稀释液以及患有横纹肌溶解症马匹的血浆进行了检测。在3、6、12和24小时后，根据凝胶弯月面处形成沉淀的迁移距离绘制标准曲线。在琼脂中抗血清1:20稀释时形成的沉淀线最清晰。标准曲线是非线性的，血浆肌红蛋白在肌红蛋白含量为2微克/毫升或更高时可被检测到。在约25℃下24小时读取时，该检测效果最佳，误差为5.6%。含有琼脂的试管可在4℃下保存6个月而不影响检测准确性。肌红蛋白对骨骼肌或心肌的特异性，以及其在肌肉坏死后从血清中的快速清除，使其非常适合用于评估急性肌肉损伤以及检测马匹在运动试验后发生横纹肌溶解症的易感性。
