Zhang Ting, Xu Luo-shan, Wang Zheng-tao, Zhou Kai-ya, Zhang Ning, Shi Yong-feng
Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China.
Yao Xue Xue Bao. 2005 Aug;40(8):728-33.
To establish a simple method for molecular identification of original plants of D. chrysanthum and D. fimbriatum using molecular marker rDNA ITS region.
Restriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D. chrysanthum and its morphologically allied species were digested at 37 degrees C by Cla I and Apa LI, those of D. fimbriatum and its morphologically allied species were digested by Sph I.
D. chrysanthum, D. fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of "Shihu" collected in markets was identified by this method.
The results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine "Shihu".
建立一种利用分子标记rDNA ITS区域对金钗石斛和流苏石斛原植物进行分子鉴定的简便方法。
采用PCR-RFLP方法获得ITS片段的限制性图谱。金钗石斛及其形态学近缘种的PCR产物用Cla I和Apa LI在37℃下消化，流苏石斛及其形态学近缘种的PCR产物用Sph I消化。
通过PCR-RFLP预测的限制性图谱可鉴定金钗石斛、流苏石斛及其形态学近缘种。用该方法鉴定了市场上采集的25份新鲜“石斛”样品的植物来源。
结果表明，rDNA ITS区域的PCR-RFLP分析是一种可行、简便且廉价的确定中药“石斛”植物来源的方法。
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