Ding Xiao-yu, Wang Zheng-tao, Xu Hong, Xu Luo-san, Zhou Kai-ya
Department of Pharmacognosy, China Pharmaceutical University, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Yao Xue Xue Bao. 2002 Jul;37(7):567-73.
To establish the whole rDNA ITS region sequence database of various Dendrobium species of "Fengdou" and to authenticate exactly the inspected species of "Fengdou".
The rDNA ITS regions of various Dendrobium species of "Fengdou" were amplified and sequenced. The database of their rDNA ITS regions was established in order to authenticate the inspected species by means of the softwares of CLUSTRAL and MEGA which were used to analyze the rDNA ITS region.
A database of the rDNA ITS sequences of 21 species of Dendrobium has been established. The notable and stable differences of the interspecies of the rDNA ITS regions have been demonstrated. The numbers of transitions and transversions among 21 species are 11-122. The variable sites are 341 while the informative sites are 195. The ITS sequence differences between the outgroup species (Pholidota yunnanensis) and species of "Fengdou" are obvious. The numbers of transitions and transversions are 131-161. The population differences of the rDNA ITS region of various species of "Fengdou" are very small (0-6).
On the basis of the database of various Dendrobium species of "Fengdou" and two genetics software, the botanical origin of the inspected species of "Fengdou" has been authenticated successfully by sequencing the rDNA ITS regions.
建立“枫斗”石斛属多种植物的核糖体DNA内转录间隔区（rDNA ITS）全序列数据库，准确鉴定“枫斗”的送检种类。
对“枫斗”石斛属多种植物的rDNA ITS区进行扩增和测序，利用CLUSTAL和MEGA软件建立其rDNA ITS区数据库，分析rDNA ITS区以鉴定送检种类。
建立了21种石斛的rDNA ITS序列数据库，显示出rDNA ITS区种间有显著且稳定的差异。21种之间的转换和颠换数为11 - 122，可变位点为341个，信息位点为195个。外类群物种（云南石仙桃）与“枫斗”物种之间的ITS序列差异明显，转换和颠换数为131 - 161。“枫斗”各物种rDNA ITS区的群体差异非常小（0 - 6）。
基于“枫斗”石斛属多种植物数据库及两个遗传学软件，通过对rDNA ITS区测序成功鉴定了“枫斗”送检种类的植物来源。
