非氧化乙醇代谢：脂肪酸乙酯合酶III在培养神经细胞中的表达

Nonoxidative ethanol metabolism: expression of fatty acid ethyl ester synthase-III in cultured neural cells.

作者信息

Isenberg K E, Bora P S, Zhou X, Wu X, Moore B W, Lange L G

机构信息

Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110.

出版信息

Biochem Biophys Res Commun. 1992 Jun 30;185(3):938-43. doi: 10.1016/0006-291x(92)91717-5.

DOI:10.1016/0006-291x(92)91717-5

PMID:1627145

Abstract

Alcohol metabolism in the human brain has been characterized as essentially nonoxidative in nature, with the esterification of ethanol with fatty acids via fatty acid ethyl ester synthase. This pathway of ethanol metabolism is related to end organ damage in the brain but the neural cell type expressing FAEES has not been identified. In this study human and rodent neuroblastoma and glioma cell lines are assayed for fatty acid ethyl ester synthase activity. Cells with neuronal properties demonstrated higher activity than glioma cell lines. We confirmed the presence of the mRNA for one type of synthase, fatty acid ethyl ester synthase-III in three neuronal cell lines--N1E115 cells, PC12 cells, and SK-N-MC cells. These results support the hypothesis that FAEES activity is expressed chiefly in cells with neuronal properties and suggest that non-oxidative ethanol metabolism is potentially related to the toxic effect of ethanol on the human brain.

摘要

人类大脑中的酒精代谢本质上具有非氧化特性，通过脂肪酸乙酯合酶使乙醇与脂肪酸发生酯化反应。这种乙醇代谢途径与大脑中的终末器官损伤有关，但表达脂肪酸乙酯合酶（FAEES）的神经细胞类型尚未确定。在本研究中，对人和啮齿动物的神经母细胞瘤及胶质瘤细胞系进行了脂肪酸乙酯合酶活性检测。具有神经元特性的细胞显示出比胶质瘤细胞系更高的活性。我们证实了在三种神经元细胞系——N1E115细胞、PC12细胞和SK-N-MC细胞中存在一种合酶的mRNA，即脂肪酸乙酯合酶-III。这些结果支持了以下假设：FAEES活性主要在具有神经元特性的细胞中表达，并表明非氧化乙醇代谢可能与乙醇对人类大脑的毒性作用有关。