De Nardin E, Radel S J, Lewis N, Genco R J, Hammarskjöld M
Department of Oral Biology, State University of New York, Buffalo.
Biochem Int. 1992 Mar;26(3):381-7.
The neutrophil FMLP receptor is involved in activation and subsequent response to certain chemotactic stimuli. The normal receptor has been reported to consist of several components, ranging in size from 43-94 kDa, and to contain both high and low affinity states. However, limited information is available on the gene/s which encode for the receptor. In this study, we have generated oligonucleotide probes derived from a published cDNA sequence encoding for one of the components of the FMLP receptor, and used these probes to amplify genomic DNA from HL-60 cells as well as normal human neutrophils, using the polymerase chain reaction. Such procedure resulted in the amplification of a single, approximately 1 kb fragment of genomic DNA identical in sequence to the cDNA described in the literature for one of the isoforms of the receptor. This finding supports the notion that the human FMLP receptor is encoded by at least one, intronless gene.
中性粒细胞FMLP受体参与激活过程以及随后对某些趋化刺激的反应。据报道，正常受体由几个大小在43 - 94 kDa之间的成分组成，并包含高亲和力和低亲和力状态。然而，关于编码该受体的基因的信息有限。在本研究中，我们从已发表的编码FMLP受体一个成分的cDNA序列中生成了寡核苷酸探针，并使用这些探针通过聚合酶链反应从HL-60细胞以及正常人中性粒细胞中扩增基因组DNA。该程序导致扩增出一个单一的、约1 kb的基因组DNA片段，其序列与文献中描述的该受体一种同工型的cDNA相同。这一发现支持了人类FMLP受体由至少一个无内含子基因编码的观点。
