Thomas E, Papay R S, Goldberg V M, Malemud C J
Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Biochem Int. 1992 Jun;27(1):165-72.
A low buoyant density fraction (A4) was isolated from human cartilage by CsCl density gradient ultracentrifugation. This fraction contained a hydrodynamically small proteoglycan (Kav, 0.74 on Sepharose CL-2B) that reacted with monoclonal antibody 12/20/1C6 specific for the hyaluronic acid binding region (G1 globe) of the large aggregating high-density proteoglycan isolated from many animal cartilages. Despite the presence of the hyaluronic acid binding region, this small proteoglycan did not form proteoglycan aggregates with hyaluronan, not even in the presence of link protein.
通过氯化铯密度梯度超速离心从人软骨中分离出一个低浮力密度组分（A4）。该组分含有一种流体动力学上较小的蛋白聚糖（在琼脂糖CL-2B上的分配系数Kav为0.74），它能与单克隆抗体12/20/1C6发生反应，该抗体对从多种动物软骨中分离出的大型聚集高密度蛋白聚糖的透明质酸结合区域（G1球体）具有特异性。尽管存在透明质酸结合区域，但这种小蛋白聚糖即使在有连接蛋白存在的情况下也不会与透明质酸形成蛋白聚糖聚集体。
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