Palmer J L, Bertone A L, Malemud C J, Carter B G, Papay R S, Mansour J
Department of Veterinary Clinical Sciences, Ohio State University, Columbus 43210, USA.
Am J Vet Res. 1995 Dec;56(12):1570-6.
The relevance of site and the influence of exercise on third carpal articular cartilage proteoglycan (PG) were assessed in 16 horses. Six horses were exercised (exercised group) for 30 minutes, 3 times/wk, for 6 weeks. The other 10 horses (nonexercised group) were housed in box stalls for the same 6-week period. At week 6, articular cartilage from the proximal surface of the right third carpal bone was harvested and cultured with radioactive sulfate to label newly synthesized PG. Endogenous PG was measured by use of a uronic acid assay. Newly synthesized and endogenous PG were characterized by use of Sepharose CL-2B chromatography, composite gel electrophoresis, and/or immunoblot analysis with monoclonal antibody 1C6 directed against the hyaluronic acid-binding region on PG. There was a significant (P = 0.0002) effect of exercise, but not site, on newly synthesized PG, which was increased in the exercised horses, compared with the nonexercised horses at the end of the 6-week study period. The increase in newly synthesized PG was not reflected in the existing cartilage matrix as there was no significant difference between groups in endogenous PG. However, there was a significant (P = 0.01) effect of site on endogenous PG, with the nest of sites located in the palmar aspect of the radial facet containing a greater concentration of endogenous PG than the nests of sites located on the dorsal aspect of the radial facet or all sites on the intermediate facet. Most newly synthesized PG in both groups consisted of hydrodynamically small PG monomers. However, there was a change in the profile of newly synthesized PG at some sites in the exercised horses to include an early elution peak on Sepharose CL-2B, which may contain aggregating PG. All sites in both groups contained a diverse population of endogenous large and small PG on toluidine blue-stained composite gels that reacted with monoclonal antibody 1C6, indicating the potential to bind to hyaluronic acid.
在16匹马中评估了部位的相关性以及运动对第三腕关节软骨蛋白聚糖（PG）的影响。6匹马进行运动（运动组），每周3次，每次30分钟，共6周。另外10匹马（非运动组）在同一6周期间饲养在厩舍中。在第6周时，采集右侧第三腕骨近端表面的关节软骨，并用放射性硫酸盐培养以标记新合成的PG。通过糖醛酸测定法测量内源性PG。新合成的和内源性PG通过琼脂糖CL-2B色谱法、复合凝胶电泳和/或用针对PG上透明质酸结合区域的单克隆抗体1C6进行免疫印迹分析来表征。在6周研究期结束时，运动对新合成的PG有显著影响（P = 0.0002），但对部位没有影响，与非运动马相比，运动马的新合成PG增加。新合成PG的增加并未反映在现有的软骨基质中，因为两组在内源性PG方面没有显著差异。然而，部位对内源性PG有显著影响（P = 0.01），位于桡骨关节面掌侧的部位巢所含内源性PG浓度高于位于桡骨关节面背侧的部位巢或中间关节面的所有部位。两组中大多数新合成的PG由流体动力学上较小的PG单体组成。然而，运动马某些部位新合成PG的图谱发生了变化，在琼脂糖CL-2B上出现了一个早期洗脱峰，可能含有聚集的PG。两组的所有部位在甲苯胺蓝染色的复合凝胶上都含有多种内源性大、小PG群体，它们与单克隆抗体1C6反应，表明具有与透明质酸结合的潜力。
