β-隐黄质与硫酸锌对大鼠股骨组织骨成分的体外协同作用：锌独特的合成代谢效应

Synergistic effect of beta-cryptoxanthin and zinc sulfate on the bone component in rat femoral tissues in vitro: the unique anabolic effect with zinc.

作者信息

Uchiyama Satoshi, Ishiyama Kaori, Hashimoto Ken, Yamaguchi Masayoshi

机构信息

Laboratory of Endocrinology and Molecular Metabolism, Graduate School of Nutritional Sciences, University of Shizuoka, Japan.

出版信息

Biol Pharm Bull. 2005 Nov;28(11):2142-5. doi: 10.1248/bpb.28.2142.

DOI:10.1248/bpb.28.2142

PMID:16272706

Abstract

The effect of the combination of beta-cryptoxanthin and zinc sulfate (zinc) on bone components in the femoral-diaphyseal and -metaphyseal tissues of young rats in vitro was investigated. Bone tissues were cultured for 48 h in a serum-free Dulbecco's modified Eagle's medium containing either vehicle, beta-cryptoxanthin (10(-9)-10(-7) M) or zinc sulfate (10(-6)-10(-4) M). The presence of beta-cryptoxanthin (10(-9) M) or zinc (10(-6) M) did not have a significant effect on calcium content in the femoral-diaphyseal or -metaphyseal tissues. However, culture which combined beta-cryptoxanthin (10(-9) M) and zinc (10(-6) M) caused a significant increase in calcium content in the femoral-diaphyseal and -metaphyseal tissues. Such an effect was not observed by the combination of beta-cryptoxanthin (10(-9) M) plus genistein (10(-6) M) or menaquinone-7 (10(-6) M), or zinc (10(-6) M) plus genistein (10(-6) M) or menaquinone-7 (10(-6) M). Also, the combination of beta-cryptoxanthin (10(-9) M) plus zinc (10(-6) M) caused a remarkable increase in alkaline phosphatase activity and deoxyribonucleic acid (DNA) in the femoral-diaphyseal and -metaphyseal tissues, while their application alone did not have an effect on the enzyme activity or DNA content in the femoral tissues. The effect of the combination of beta-cryptoxanthin (10(-9) M) plus zinc (10(-6) M) in increasing calcium content, alkaline phosphatase activity, and DNA content in the femoral-diaphyseal and -metaphyseal tissues was completely prevented in the presence of cycloheximide (10(-6) M), an inhibitor of protein synthesis, or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DBR), an inhibitor of transcriptional activity. This study demonstrates that the combination of beta-cryptoxanthin and zinc at a lower concentration has a synergistic effect on bone components in vitro.

摘要

研究了β-隐黄质与硫酸锌（锌）组合对幼鼠股骨干和干骺端组织骨成分的体外影响。将骨组织在含有赋形剂、β-隐黄质（10⁻⁹ - 10⁻⁷ M）或硫酸锌（10⁻⁶ - 10⁻⁴ M）的无血清杜尔贝科改良伊格尔培养基中培养48小时。β-隐黄质（10⁻⁹ M）或锌（10⁻⁶ M）的存在对股骨干或干骺端组织中的钙含量没有显著影响。然而，β-隐黄质（10⁻⁹ M）和锌（10⁻⁶ M）联合培养导致股骨干和干骺端组织中的钙含量显著增加。β-隐黄质（10⁻⁹ M）加染料木黄酮（10⁻⁶ M）或甲萘醌-7（10⁻⁶ M），或锌（10⁻⁶ M）加染料木黄酮（10⁻⁶ M）或甲萘醌-7（10⁻⁶ M）的组合未观察到这种效果。此外，β-隐黄质（10⁻⁹ M）加锌（10⁻⁶ M）的组合导致股骨干和干骺端组织中的碱性磷酸酶活性和脱氧核糖核酸（DNA）显著增加，而单独应用它们对股骨组织中的酶活性或DNA含量没有影响。在蛋白质合成抑制剂环己酰亚胺（10⁻⁶ M）或转录活性抑制剂5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DBR）存在的情况下，β-隐黄质（10⁻⁹ M）加锌（10⁻⁶ M）组合在增加股骨干和干骺端组织中钙含量、碱性磷酸酶活性和DNA含量方面的作用完全被阻断。本研究表明，较低浓度的β-隐黄质和锌组合在体外对骨成分具有协同作用。