Okano Takayuki, Nakagawa Takayuki, Endo Tsuyoshi, Kim Tae-Soo, Kita Tomoko, Tamura Tetsuya, Matsumoto Masahiro, Ohno Tsunehisa, Sakamoto Tatsunori, Iguchi Fukuichiro, Ito Juichi
Department of Otolaryngology-Head and Neck Surgery, Kyoto University Graduate School of Medicine, Shogoin, Kyoto, Japan.
Neuroreport. 2005 Nov 28;16(17):1919-22. doi: 10.1097/01.wnr.0000187628.38010.5b.
This study aimed to evaluate the potential of embryonic stem cell-derived neural progenitors for use as transplants for the replacement of the auditory primary neurons, spiral ganglion neurons. Mouse embryonic stem cell-derived neural progenitors were implanted into the base of the cochlear modiolus of normal or deafened guinea pigs, which contains spiral ganglion neurons and cochlear nerve fibers. Histological analysis demonstrated the survival and neural differentiation of transplants in the cochlear modiolus and active neurite outgrowth of transplants toward host peripheral or central auditory systems. Functional assessments indicated the potential of transplanted embryonic stem cell-derived neural progenitors to elicit the functional recovery of damaged cochleae. These findings support the hypothesis that transplantation of embryonic stem cell-derived neural progenitors can contribute to the functional restoration of spiral ganglion neurons.
本研究旨在评估胚胎干细胞来源的神经前体细胞作为移植材料替代听觉初级神经元——螺旋神经节神经元的潜力。将小鼠胚胎干细胞来源的神经前体细胞植入正常或耳聋豚鼠的蜗轴底部,蜗轴包含螺旋神经节神经元和耳蜗神经纤维。组织学分析表明,移植细胞在蜗轴内存活并发生神经分化,且移植细胞的活跃神经突向宿主外周或中枢听觉系统生长。功能评估表明,移植的胚胎干细胞来源的神经前体细胞具有促使受损耳蜗功能恢复的潜力。这些发现支持了以下假说:胚胎干细胞来源的神经前体细胞移植有助于螺旋神经节神经元的功能恢复。