Nishimura Koji, Nakagawa Takayuki, Ono Kazuya, Ogita Hideaki, Sakamoto Tatsunori, Yamamoto Norio, Okita Keisuke, Yamanaka Shinya, Ito Juichi
Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
Neuroreport. 2009 Sep 23;20(14):1250-4. doi: 10.1097/WNR.0b013e32832ff287.
This study examined the potential of induced pluripotent stem (iPS) cells for use as a source of transplants for the restoration of auditory spiral ganglion neurons. We monitored neurite outgrowth from iPS cell-derived neural progenitors toward cochlear hair cells ex vivo, and followed their survival and fates after transplantation into mouse cochleae in vivo. Neurons derived from iPS cells projected neurites toward cochlear hair cells. The settlement of iPS cell-derived neurons was observed 1 week after transplantation into the cochlea. Some transplants expressed vesicular glutamate transporter 1, which is a marker for glutamatergic neurons. These findings indicate that iPS cells can be used as a source of transplants for the regeneration of spiral ganglion neurons.
本研究探讨了诱导多能干细胞（iPS细胞）作为移植来源用于恢复听觉螺旋神经节神经元的潜力。我们在体外监测了iPS细胞来源的神经祖细胞向耳蜗毛细胞的神经突生长，并在体内将其移植到小鼠耳蜗后跟踪它们的存活和命运。iPS细胞衍生的神经元向耳蜗毛细胞伸出神经突。将其移植到耳蜗1周后观察到iPS细胞衍生神经元的定植。一些移植细胞表达了囊泡谷氨酸转运体1，这是谷氨酸能神经元的标志物。这些发现表明，iPS细胞可作为螺旋神经节神经元再生的移植来源。
