Nakase Minoru, Okumura Kenya, Kamei Takahiko, Nakamura Shinnosuke, Inui Madoka, Tagawa Toshiro
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
Oncol Rep. 2005 Dec;14(6):1487-91.
The transfection efficiency of cationic liposomes varies according to cell type, but the specific cellular characteristics that affect transfection efficiency have not yet been defined. We investigated whether the transfection efficiency of cationic liposomes correlates with cell proliferation activity or cell membrane potential in oral malignant melanoma (HMG) and oral osteosarcoma cell lines (HOSM-1 and HOSM-2). The cell membrane potential was assessed by uptake of a cationic probe. Three oral tumor cell lines were exposed to a cationic liposome complexed with a beta-galactosidase expression plasmid, and beta-galactosidase expression was compared. Cell proliferation was about 2-fold higher in HOSM-1 cells than in HMG cells. The cell membrane potential in HMG and HOSM-1 cells was comparable, while the membrane potential in HOSM-2 cells was 1.6-fold higher. beta-galactosidase expression was measured by X-Gal staining in 7.0% of HMG, 17.0% of HOSM-1 and 11.5% of HOSM-2 cells. The present study demonstrates that gene therapy with cationic liposomes may be a promising new strategy for treatment of oral malignant melanoma and osteosarcoma. In addition, the transfection efficiency of cationic liposomes appears to be influenced by cell proliferation activity, but not cell membrane potential.
阳离子脂质体的转染效率因细胞类型而异，但影响转染效率的具体细胞特性尚未明确。我们研究了阳离子脂质体的转染效率与口腔恶性黑色素瘤（HMG）以及口腔骨肉瘤细胞系（HOSM - 1和HOSM - 2）的细胞增殖活性或细胞膜电位是否相关。通过摄取阳离子探针评估细胞膜电位。将三种口腔肿瘤细胞系暴露于与β - 半乳糖苷酶表达质粒复合的阳离子脂质体中，并比较β - 半乳糖苷酶的表达情况。HOSM - 1细胞中的细胞增殖比HMG细胞高约2倍。HMG和HOSM - 1细胞的细胞膜电位相当，而HOSM - 2细胞中的膜电位高1.6倍。通过X - Gal染色检测到，7.0%的HMG细胞、17.0%的HOSM - 1细胞和11.5%的HOSM - 2细胞中有β - 半乳糖苷酶表达。本研究表明，阳离子脂质体基因治疗可能是治疗口腔恶性黑色素瘤和骨肉瘤的一种有前景的新策略。此外，阳离子脂质体的转染效率似乎受细胞增殖活性影响，而不受细胞膜电位影响。
