人类散发性结直肠癌中生长抑制因子1的基因改变与表达
Genetic alterations and expression of inhibitor of growth 1 in human sporadic colorectal cancer.
作者信息
Chen Li-Sheng, Wei Jian-Bao, Zhou Yong-Chun, Zhang Sen, Liang Jun-Lin, Cao Yun-Fei, Tang Zong-Jiang, Zhang Xiao-Long, Gao Feng
机构信息
Department of Coloproctological Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Province, China.
出版信息
World J Gastroenterol. 2005 Oct 21;11(39):6120-4. doi: 10.3748/wjg.v11.i39.6120.
AIM
To explore the effect and significance of inhibitor of growth 1 (ING1) gene in carcinogenesis and progression of human sporadic colorectal cancer.
METHODS
mRNA expression, mutation, and loss of heterozygosity (LOH) of ING1 gene in 35 specimens of sporadic colorectal cancer tissues and the matched normal mucous membrane tissues were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), PCR-single strain conformation polymorphism (PCR-SSCP) and PCR-simple sequence length polymorphism (PCR-SSLP) using microsatellite markers, respectively.
RESULTS
The average ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues were significantly lower than those in normal tissues. The difference between the two mRNA splices was not significant in the matched tissues. In addition, the ratios of light intensities of p33(ING1b) and p47(ING1a) mRNA expression in the cancerous tissues of Dukes' stages C and D were significantly lower than those in cancerous tissues of Dukes' stages A and B. However, no mutation of ING1 gene was detected in all 35 cases; only 4 cases of LOH (11.4%) were found.
CONCLUSION
p33(ING1b) and p47(ING1a) mRNA expressions are closely related with the carcinogenesis and progression of human sporadic colorectal cancer. No mutation of ING1 gene is found, and there are only few LOH in sporadic colorectal cancers. These might not be the main reasons for the down regulation of ING1 expression. Its low expression may happen in transcription or post-transcription.
目的
探讨生长抑制因子1（ING1）基因在人类散发性结直肠癌发生发展中的作用及意义。
方法
分别采用半定量逆转录聚合酶链反应（RT-PCR）、聚合酶链反应-单链构象多态性（PCR-SSCP）以及利用微卫星标记的聚合酶链反应-简单序列长度多态性（PCR-SSLP）检测35例散发性结直肠癌组织及其配对的正常黏膜组织中ING1基因的mRNA表达、突变及杂合性缺失（LOH）情况。
结果
癌组织中p33（ING1b）和p47（ING1a）mRNA表达的平均光密度比值显著低于正常组织。在配对组织中，两种mRNA剪接体之间的差异不显著。此外，Dukes分期C期和D期癌组织中p33（ING1b）和p47（ING1a）mRNA表达的光密度比值显著低于Dukes分期A期和B期的癌组织。然而，35例病例中均未检测到ING1基因的突变；仅发现4例杂合性缺失（11.4%）。
结论
p33（ING1b）和p47（ING1a）mRNA表达与人类散发性结直肠癌的发生发展密切相关。未发现ING1基因的突变，散发性结直肠癌中杂合性缺失也很少见。这些可能不是ING1表达下调的主要原因。其低表达可能发生在转录或转录后阶段。
Zotero 插件