[胃癌组织及癌前组织中p33(ING1)基因的表达与突变]

[p33(ING1) gene expression and mutation in stomach cancer tissues and precarcinomatous tissues].

作者信息

Ding Hou-zhong, Yang Dong, Wu Fu-rong, Li Hai, Feng Kun, Zheng Si-jie, Ni Can-rong, Yu Guan-zhen

机构信息

Kunshan No. 1 People's Hospital, Kunshan Jiangsu, 215300, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2003 Feb 25;83(4):320-3.

PMID:12812652

Abstract

OBJECTIVE

To analyze the relationship of p33(ING1) gene expression and p33(ING1) exon-2 mutation to the pathogenesis, development and consequence of stomach cancer.

METHODS

Envision immunohistochemical method was utilized to detect the p33(ING1) expression in 103 specimens of stomach cancer, 36 specimens of stomach mucosal atypical hyperplasia, and 32 specimens of normal stomach mucosa. PCR-SSCP was utilized to detect p33(ING1) exon-2 mutation in stomach cancer tissues.

RESULTS

The p33(ING1) expression rate in stomach cancer was 54.4% (56/103), significantly lower than that in precarcinomatous tissues (94.4%, 34/36, P < 0.01) and that in normal tissues (100%, 32/32, P < 0.01). The p33(ING1) expression in stomach cancer was related to tumor growth, distant metastasis and tumor differentiation (all P < 0.05). p33(ING1) gene exon-2 mutation was detected in 3 cases of stomach cancer tissues (12%, 3/25), and not in other tissues by PCR-SSCP method.

CONCLUSION

p33(ING1) low expression, and gene p33(ING1) exon-2 mutation may play an important role in the pathogenesis, development and consequence of stomach cancer.

摘要

目的

分析p33(ING1)基因表达及p33(ING1)外显子2突变与胃癌发生、发展及预后的关系。

方法

采用Envision免疫组化法检测103例胃癌标本、36例胃黏膜非典型增生标本及32例正常胃黏膜标本中p33(ING1)的表达。采用聚合酶链反应-单链构象多态性(PCR-SSCP)法检测胃癌组织中p33(ING1)外显子2突变情况。

结果

胃癌组织中p33(ING1)表达率为54.4%(56/103)，显著低于癌前组织(94.4%，34/36，P<0.01)及正常组织(100%，32/32，P<0.01)。胃癌组织中p33(ING1)表达与肿瘤生长、远处转移及肿瘤分化均有关(P均<0.05)。采用PCR-SSCP法在25例胃癌组织中检测到3例p33(ING1)基因外显子2突变(12%，3/25)，其他组织未检测到突变。