Terman A
Department of Genetics and Animal Breeding, Agricultural University of Szczecin, Szczecin, Poland.
J Anim Breed Genet. 2005 Dec;122(6):400-4. doi: 10.1111/j.1439-0388.2005.00547.x.
The aim of the experiment was to use the DNA mutations in the PRLR and LEP genes to determine associations between the genotype and litter size in Polish Large White x Landrace sows. Reproductive traits investigated were: total number of piglets born (TNB), number of piglets born alive (NBA) and number of piglets weaned. The polymorphism in PRLR and LEP genes was detected using the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method, with specific primers and the restriction enzymes AluI and HinfI respectively. Two different alleles of PRLR and LEP gene were identified: alleles A (0.62) and B (0.38) of the PRLR gene and alleles C (0.10) and T (0.90) of the LEP gene. The relationships between the PRLR and LEP genotypes and TBN, NBA and NW were analysed. The analysis showed, in first parity sows, statistically significant (p < or = 0.01) differences between sows carrying different PRLR genotypes. In later parities, sows with the AA genotype still had the largest litter size compared with AB and BB sows, but the difference was statistically not significant. Analysis of the interaction PARITY x PRLR showed small and statistically not significant differences. The analysis of relationship between different LEP genotypes and TNB, NBA, NW showed small and statistically non-significant differences.
该实验的目的是利用催乳素受体（PRLR）基因和瘦素（LEP）基因中的DNA突变，来确定波兰大白猪×长白猪母猪的基因型与产仔数之间的关联。所研究的繁殖性状包括：总产仔数（TNB）、产活仔数（NBA）和断奶仔猪数。分别使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，通过特异性引物以及限制性内切酶AluI和HinfI，检测PRLR和LEP基因的多态性。鉴定出PRLR和LEP基因的两个不同等位基因：PRLR基因的等位基因A（0.62）和B（0.38），以及LEP基因的等位基因C（0.10）和T（0.90）。分析了PRLR和LEP基因型与TBN、NBA和NW之间的关系。分析表明，在头胎母猪中，携带不同PRLR基因型的母猪之间存在统计学显著差异（p≤0.01）。在经产母猪中，与AB和BB基因型母猪相比，AA基因型母猪的产仔数仍然最多，但差异无统计学意义。对胎次×PRLR交互作用的分析显示差异较小且无统计学意义。对不同LEP基因型与TNB、NBA、NW之间关系的分析显示差异较小且无统计学意义。
Zotero 插件