Thiry M, Goessens G
Laboratory of Cell and Tissue Biology, University of Liège, Belgium.
Int J Dev Biol. 1992 Mar;36(1):139-42.
We have investigated the DNA distribution within the rat oocyte nucleolus during the early stages of follicular growth by means of the in situ terminal deoxynucleotidyl transferase method. In the fibrillogranular nucleolus, label is visualized on small clumps of peri- and intranucleolar chromatin. Such labeled clumps are frequently observed inside the interstices surrounding the fibrillar centers. Label is also consistently found in the fibrillar centers whereas the dense fibrillar component and the granular component are devoid of gold particles. These results contradict earlier data but conform with other recent immunocytochemical observations, obtained in nucleoli of a variety of somatic cell types, concerning the correlation between structure and function in the nucleolus.
我们通过原位末端脱氧核苷酸转移酶法研究了卵泡生长早期大鼠卵母细胞核仁内的DNA分布。在纤维颗粒状核仁中，标记物可见于核仁周围和核仁内染色质的小团块上。在围绕纤维中心的间隙内经常观察到这种标记的团块。在纤维中心也始终发现有标记，而致密纤维成分和颗粒成分则没有金颗粒。这些结果与早期数据相矛盾，但与最近在各种体细胞类型的核仁中获得的其他免疫细胞化学观察结果一致，这些观察结果涉及核仁结构与功能之间的相关性。
