Negrutiu I, Hinnisdaels S, Cammaerts D, Cherdshewasart W, Gharti-Chhetri G, Jacobs M
Laboratory of Plant Genetics, Vrije Universiteit Brussel, Belgium.
Int J Dev Biol. 1992 Mar;36(1):73-84.
Protoplasts have usually been presented as a methodological tool. Even as such, they make possible an impressive array of applications in plant biology. Here we report on the use of protoplast-derived selectable markers in the study of several disturbed genetic systems with obvious effects on plant development: (1) auxotrophic mutants and the control of amino acid biosynthesis and transport in vegetative and reproductive tissues; (2) introgression of alien genetic information across phylogenetic boundaries by protoplast fusion, a consequence of controlled dedifferentiation-redifferentiation processes and attenuated incompatibility reactions in cultured cells; (3) expression (in)stability of foreign genes in transgenic plants during successive meiotic generations and in crosses between independent transformants.
原生质体通常被视为一种方法学工具。即便如此，它们在植物生物学中仍能实现一系列令人瞩目的应用。在此，我们报告了原生质体衍生的选择标记在几个对植物发育有明显影响的受干扰遗传系统研究中的应用：（1）营养缺陷型突变体以及营养和生殖组织中氨基酸生物合成与转运的调控；（2）通过原生质体融合将外源遗传信息跨越系统发育界限进行渐渗，这是培养细胞中受控的去分化 - 再分化过程以及减弱的不亲和反应的结果；（3）外源基因在转基因植物连续减数分裂世代以及独立转化体之间杂交过程中的表达（不）稳定性。
