Hsu D H, Moore K W, Spits H
Department of Immunology, DNAX Research Institute, Palo Alto, CA 94304.
Int Immunol. 1992 May;4(5):563-9. doi: 10.1093/intimm/4.5.563.
Culture of human peripheral blood mononuclear cells (PBMC) with IL-2 stimulates synthesis of cytokines and generation of lymphokine-activated killer (LAK) activity. Both IL-4 and IL-10 [cytokine synthesis inhibitory factor (CSIF)] inhibit IL-2-induced synthesis of IFN-gamma and tumor necrosis factor (TNF)-alpha by human PBMC. However, unlike IL-4, IL-10 inhibits neither IL-2-induced proliferation of PBMC and fresh natural killer (NK) cells, nor IL-2-induced LAK activity. Moreover, IL-4 inhibits IL-2-induced IFN-gamma synthesis by purified fresh NK cells, while in contrast the inhibitory effect of IL-10 is mediated by CD14+ cells (monocytes/macrophages). IL-10 inhibits TNF-alpha synthesis by monocytes or monocytes plus NK cells, but not by NK cells alone. These results suggest that IL-4 and IL-10 act on NK cells via distinct pathways, and that IL-2-induced cytokine synthesis and LAK activity are regulated via different mechanisms.
用人外周血单个核细胞（PBMC）与白细胞介素-2（IL-2）共培养，可刺激细胞因子的合成并产生淋巴因子激活的杀伤细胞（LAK）活性。白细胞介素-4（IL-4）和白细胞介素-10 [细胞因子合成抑制因子（CSIF）] 均可抑制人PBMC由IL-2诱导的γ干扰素（IFN-γ）和肿瘤坏死因子（TNF）-α的合成。然而，与IL-4不同，IL-10既不抑制IL-2诱导的PBMC和新鲜自然杀伤（NK）细胞的增殖，也不抑制IL-2诱导的LAK活性。此外，IL-4可抑制纯化的新鲜NK细胞由IL-2诱导的IFN-γ合成，而与之相反，IL-10的抑制作用是由CD14⁺细胞（单核细胞/巨噬细胞）介导的。IL-10可抑制单核细胞或单核细胞加NK细胞产生TNF-α，但对单独的NK细胞无此作用。这些结果表明，IL-4和IL-10通过不同途径作用于NK细胞，且IL-2诱导的细胞因子合成和LAK活性是通过不同机制进行调节的。
