Hong S J, Woo H C, Han J H, Kim H J
Department of Parasitology, College of Medicine, Gyeongsang National University, Chinju, Korea.
Kisaengchunghak Chapchi. 1992 Jun;30(2):141-5. doi: 10.3347/kjp.1992.30.2.141.
A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E.C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T. in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were 47.86 x 10(0.87) logM.C.T.S. in A. lumbricoides, 41.69 x 10(0.82) logM.C.T.S. in T. trichiura and 63.10 x 10(0.85) logM.C.T.S. in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.
总共制备了197份粪便标本,采用改良加藤氏透明厚涂片法(M.C.T.S.)和司徒尔氏稀释虫卵计数法(D.E.C.T.)对蠕虫卵进行定量检测。评估了两种技术的相对有效性,并推导了转换函数。M.C.T.S.法对一张玻片进行显微镜检查平均所需时间为12.6分钟,D.E.C.T.法为14.6分钟。在轻度蠕虫感染病例中,M.C.T.S.法的假阴性率低于D.E.C.T.法。将M.C.T.S.法获得的计数转换为司徒尔氏稀释虫卵计数法的每克粪便虫卵数(E.P.G.)的函数,蛔虫为47.86×10(0.87)logM.C.T.S.,鞭虫为41.69×10(0.82)logM.C.T.S.,华支睾吸虫为63.10×10(0.85)logM.C.T.S.。结果表明,即使在蠕虫感染率较低的情况下,M.C.T.S.法在对蛔虫、鞭虫和华支睾吸虫等肠道蠕虫感染进行定量检测方面优于D.E.C.T.法。