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用于大规模粪便检查的玻璃纸厚涂片技术评估

An Evaluation Of Cellophane Thick Smear Technique For Mass Stool Examination.

作者信息

Cho Seung Yull, Lee Soon Hyung, Rim Han Jong, Seo Byong Seol

机构信息

Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

出版信息

Kisaengchunghak Chapchi. 1969 Jun;7(1):48-52. doi: 10.3347/kjp.1969.7.1.48.

Abstract

To evaluate the Kato's cellophane thick smear techcique in detection ability of various helminth ova, authors examined 1,843 nationwidely collected stool specimens by the two methods, formalin-ether technique and cellophane thick smear technique. And the results were analyzed. 1. The positive rates of following helminth ova were superior in cellophane thick smear technique to those of formalin-ether technique: Ascaris lumbricoides(62.3% : 51.1%), Taenia species (1.5% : 0.9%). The difference in A. lumbricoides ova detection is highly significant one. The overall positive rate for helminths ova was also higher in Kato's method (92.3% : 90.0%). 2. The positive fates of following helminth ova were infrior in cellophane thick smear technique to those of formalin-ether technique: Hookworm (5.8% : 14.6%), Trichostrongylus orientalis(3.2% : 17.8%), Clonorchis sinensis(8.3% : 11.0%), Metagonimus yokogawai (0.6% : 1.7%) and Hymenolepis nana(0.05% : 0.3%). The positive rate for the ova of Trichocephalus trichiurus by the two methods was incidentally same, 74.1%. 3. The causes of inferior positive rates in some helminth ova by cellophane thick smear technique were shortly discussed and suggests some opinions in improvement of detection ability.

摘要

为评估加藤氏透明厚涂片技术对各种蠕虫卵的检测能力,作者采用两种方法,即福尔马林 - 乙醚法和透明厚涂片法,对全国范围内收集的1843份粪便标本进行了检测,并对结果进行了分析。1. 以下蠕虫卵在透明厚涂片技术中的阳性率高于福尔马林 - 乙醚技术:蛔虫(62.3% : 51.1%)、带绦虫属(1.5% : 0.9%)。蛔虫卵检测的差异具有高度显著性。加藤氏法中蠕虫卵的总体阳性率也更高(92.3% : 90.0%)。2. 以下蠕虫卵在透明厚涂片技术中的阳性率低于福尔马林 - 乙醚技术:钩虫(5.8% : 14.6%)、东方毛圆线虫(3.2% : 17.8%)、华支睾吸虫(8.3% : 11.0%)、横川后殖吸虫(0.6% : 1.7%)和微小膜壳绦虫(0.05% : 0.3%)。两种方法检测鞭虫卵的阳性率偶然相同,为74.1%。3. 简要讨论了透明厚涂片技术中某些蠕虫卵阳性率较低的原因,并对提高检测能力提出了一些意见。

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