微小隐孢子虫和鼠隐孢子虫原生动物寄生虫18S rRNA基因及内部转录间隔区1的PCR克隆与核苷酸序列测定
PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasites Cryptosporidium parvum and Cryptosporidium muris.
作者信息
Cai J, Collins M D, McDonald V, Thompson D E
机构信息
Department of Microbiology, AFRC Institute of Food Research, Reading, UK.
出版信息
Biochim Biophys Acta. 1992 Jul 15;1131(3):317-20. doi: 10.1016/0167-4781(92)90032-u.
The genes encoding 18S rRNA and internal transcribed spacer 1 (1TS1) of Cryptosporidium parvum and Cryptosporidium muris were amplified from oocysts by PCR utilizing primers complementary to conserved regions of the 5' end of 18S and 5.8S rRNA. PCR products were cloned and the complete nucleotide sequences of two clones of each Cryptosporidium species were determined. The 18S rRNA genes of C. parvum and C. muris showed more than 99% sequence identity.
利用与18S和5.8S rRNA 5'端保守区域互补的引物，通过聚合酶链反应（PCR）从小隐孢子虫和鼠隐孢子虫的卵囊中扩增出编码18S核糖体RNA（rRNA）和内部转录间隔区1（ITS1）的基因。将PCR产物克隆，并测定每个隐孢子虫物种两个克隆的完整核苷酸序列。小隐孢子虫和鼠隐孢子虫的18S rRNA基因显示出超过99%的序列同一性。
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