Okafuji T, Maekawa K, Nawa K, Marumoto Y
Molecular Biology Research Laboratory, Daiichi Pharmaceutical Co., Tokyo, Japan.
Biochim Biophys Acta. 1992 Jul 15;1131(3):329-32. doi: 10.1016/0167-4781(92)90035-x.
We cloned a cDNA coding for rat protein C, which provides hybridization probes for the detection of protein C mRNA in several tissues. The cloned cDNA was 1543 bp long and contained a single open reading frame of 1383 nucleotides. The proposed rat protein C precursor contained 461 amino acid residues: a 41 amino acid preproleader sequence, and light (155 amino acids) and heavy (263 amino acids) chains joined by a Lys-Arg dipeptide. Northern blot analysis showed that the rat protein C mRNA was expressed not only in the liver, but also in the kidney.
我们克隆了编码大鼠蛋白C的cDNA，它为检测多种组织中的蛋白C mRNA提供了杂交探针。克隆的cDNA长1543 bp，包含一个1383个核苷酸的单一开放阅读框。推测的大鼠蛋白C前体包含461个氨基酸残基：一个41个氨基酸的前导肽序列，以及由一个赖氨酸-精氨酸二肽连接的轻链（155个氨基酸）和重链（263个氨基酸）。Northern印迹分析表明，大鼠蛋白C mRNA不仅在肝脏中表达，也在肾脏中表达。
