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[噬纤维菌属JL-01降解滤纸纤维素过程的研究]

[Study on the process of degrading filter paper cellulose by Sporocytophaga sp. JL-01].

作者信息

Liu Dongbo, Zhang Ying, Chen Shan, Wang Xiuran, Zhang Liping

机构信息

School of Life Science, Northeast Normal University, Changchun 130024, China.

出版信息

Wei Sheng Wu Xue Bao. 2003 Dec;43(6):776-80.

Abstract

Sporocytophaga is a kind of bacteria that can glide on the solid medium surface. It can decompose the cotton and filter paper after 6 days cultured. The sporocytophaga can only produce a low extracellular carboxymethylcellulase activity and no other cellulase activities. In this work, the process about degradation of filter paper fibre by a strain of Sporocytophaga was studied using scan electronmicroscopy. The morphological changes that Sporocytophaga sp. JI-01 grew on filter paper were observed. The study revealed the JL-01 adhered tightly to the surface of filter paper fibre or penetrated into interior of the fibre through its 2.5 microm - 4.0 microm bacilliform cells in the process of degrading. The bacilliform cells degrade the cellulose strongly and produced a mass of sticky polysaccharides. At the anaphase of cultured, the bacteria existed as a cycloidal dormancy body-sporocyst.

摘要

噬纤维菌是一种能在固体培养基表面滑行的细菌。培养6天后,它能分解棉花和滤纸。噬纤维菌仅产生低水平的细胞外羧甲基纤维素酶活性,不产生其他纤维素酶活性。在这项工作中,利用扫描电子显微镜研究了一株噬纤维菌降解滤纸纤维的过程。观察了噬纤维菌在滤纸上生长时的形态变化。研究表明,在降解过程中,JL-01通过其2.5微米至4.0微米的杆状细胞紧密附着在滤纸纤维表面或穿透到纤维内部。杆状细胞强烈降解纤维素并产生大量粘性多糖。在培养后期,细菌以摆线状休眠体——芽孢囊的形式存在。

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