Wang Hongyuan, Fan Bingquan
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Wei Sheng Wu Xue Bao. 2010 Jul;50(7):870-5.
The aim of this study was to screen straw-cellulose degrading microorganisms and to investigate their degradation ability of straw-cellulose.
The methods used to screen the high effect straw-cellulose degrading microorganism included the traditional isolation methods of straw-cellulose degrading microorganism such as holes observation method on filter paper sheet, disintegration test of filter paper scrip, hydrolysis spot diameter measurement method of CMC-Na, weight lose assay method of straw, measurement method of cellulose decomposition rate, measurement method of extracellular enzyme activity.
We isolated 3 fungi with cellulose degrading ability, of which 98MJ was identified as Penicillium oxalicum, W3 as Trichoderma sp., and W4 as Penicillium expansum. Strain W4 possessed high straw-cellulose degrading ability with straw-cellulose degrading rate of 56.3%, cellulose 59.06%, hemicellulose 78.75% and lignin 33.79% in 10 days.
Strain W4 was a cellulase-producing strain with broad development potential.
本研究旨在筛选秸秆纤维素降解微生物，并考察其对秸秆纤维素的降解能力。
用于筛选高效秸秆纤维素降解微生物的方法包括传统的秸秆纤维素降解微生物分离方法，如滤纸打孔观察法、滤纸条崩解试验、羧甲基纤维素钠水解斑直径测量法、秸秆失重测定法、纤维素分解率测定法、胞外酶活性测定法。
我们分离出3株具有纤维素降解能力的真菌，其中98MJ被鉴定为草酸青霉，W3为木霉属，W4为扩展青霉。菌株W4具有较高的秸秆纤维素降解能力，在10天内秸秆纤维素降解率为56.3%，纤维素降解率为59.06%，半纤维素降解率为78.75%，木质素降解率为33.79%。
菌株W4是一株具有广阔开发潜力的纤维素酶产生菌。
