[玉米赤霉β-微管蛋白基因的克隆及其与多菌灵抗性关系分析]

[Cloning of beta-tubulin gene from Gibberella zeae and analysis its relationship with carbendazim-resistance].

作者信息

Li Hongxia, Lu Yuejian, Wang Jianxin, Zhou Mingguo

机构信息

Key Laboratory of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture, Nanjing Agriculture University, Nanjing 210095, China.

出版信息

Wei Sheng Wu Xue Bao. 2003 Aug;43(4):424-9.

PMID:16276914

Abstract

Whole beta-tubulin genes from wild carbendazim(MBC)-sensitive isolate, field MBC-resistant isolate and induced MBC-resistant mutant of Gibberella zeae were cloned and sequenced with 3 pairs of primers. These genes have 1631bp length, including 3 introns, encoding 447 amino acid. The homology of amino acid tubulin gene of G. zeae with that of other common plant pathogenic filamentous fungi is from 95.12% approximately 99.30%. Sequence comparison among MBCs, field MBCR and induced MBCR isolates revealed there was no mutation, even one. So it can be concluded that the mechanism of MBC-resistance to G. zeae is different from other filamentous fungi caused by point mutation at amino acid position 198 or other position of beta-tubulin gene.

摘要

利用3对引物，克隆并测序了来自野生多菌灵（MBC）敏感菌株、田间MBC抗性菌株和诱导的MBC抗性突变体的玉米赤霉全β-微管蛋白基因。这些基因长度为1631bp，包含3个内含子，编码447个氨基酸。玉米赤霉氨基酸微管蛋白基因与其他常见植物病原丝状真菌的同源性约为95.12%至99.30%。MBC敏感菌株、田间MBC抗性菌株和诱导的MBC抗性菌株之间的序列比较表明，甚至没有一个突变。因此可以得出结论，玉米赤霉对MBC的抗性机制与其他丝状真菌不同，后者是由β-微管蛋白基因第198位氨基酸或其他位置的点突变引起的。