[芒果炭疽病菌β-微管蛋白基因克隆及其与多菌灵抗性的关系]

[Cloning of -tubulin gene and their correlation with conferred carbendazim resistance of Colletotrichum gloeosporioides Penz in mango].

作者信息

Zhan Ru-Lin, Zheng Fu-Cong

机构信息

Southern Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, Zhanjiang, China.

出版信息

Wei Sheng Wu Xue Bao. 2004 Dec;44(6):827-9.

PMID:16110970

Abstract

The total DNA isolated from MBC-resistant and MBC-sensitive isolates of Colletotrichum gloeosporioides Penz (C.g.M) of mango were used as templates in PCR amplification using consensus oligo nucleotide primers designed according to the known sequence data of beta-tubulin-encoding gene (tub 1 and tub2) of Colletotrichum gloeosporioides f. sp. aeschynomene (C. g. A). Only the primers designed according to C. g. A tub2 amplified specific fragments. These amplified fragments were cloned and sequenced. The results showed that these fragments have 1344bp and deduced 447 amino acid, which were highly homologous to C.g.A tub2. MBC-resistant isolates did not carry the allelic mutation at amino acid codes 198 and 200 of beta-tubulin gene in comparison with the sensitive isolates. However, the amino acid altered in codes 181, 237 and 363.

摘要

从芒果炭疽病菌（Colletotrichum gloeosporioides Penz，C.g.M）的MBC抗性和MBC敏感分离株中分离得到的总DNA，作为模板用于PCR扩增，所用的共有寡核苷酸引物是根据胶孢炭疽菌（Colletotrichum gloeosporioides f. sp. aeschynomene，C. g. A）β-微管蛋白编码基因（tub 1和tub2）的已知序列数据设计的。只有根据C. g. A tub2设计的引物扩增出了特异性片段。这些扩增片段被克隆并测序。结果表明，这些片段有1344bp，推导的氨基酸有447个，与C.g.A tub2高度同源。与敏感分离株相比，MBC抗性分离株在β-微管蛋白基因的第198和200位氨基酸编码处没有发生等位基因突变。然而，在第181、237和363位氨基酸编码处发生了改变。