Zhu Weiyun, Yao Wen, Mao Shengyong
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wei Sheng Wu Xue Bao. 2003 Aug;43(4):503-8.
PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor the development of bacterial community in faeces of 12 weaning piglets. The regions V6 to V8 of the 16S rDNA of faecal bacteria were amplified. DGGE profiles of the PCR amplicons were compared by similarity analysis. Results revealed simple DGGE profiles for faecal samples from piglets on the first day of weaning and piglets from the same sow showed similar DGGE profiles. After weaning as the piglets grew DGGE profiles became complicated and diverse. Each individual piglet had its unique DGGE profile, with low similarity between piglets. The source of sow and the diet showed no apparent effect on the DGGE profiles. For the piglets fed a diet containing oliogofructose, DGGE similarity analysis showed bacterial community in the faeces developed fast during the first week and relatively slow later after weaning.
采用聚合酶链反应(PCR)和变性梯度凝胶电泳(DGGE)技术监测12头断奶仔猪粪便中细菌群落的发育情况。扩增粪便细菌16S rDNA的V6至V8区域。通过相似性分析比较PCR扩增产物的DGGE图谱。结果显示,断奶第一天仔猪粪便样本的DGGE图谱简单,同一母猪的仔猪表现出相似的DGGE图谱。断奶后随着仔猪生长,DGGE图谱变得复杂多样。每头仔猪都有其独特的DGGE图谱,仔猪之间的相似性较低。母猪来源和日粮对DGGE图谱无明显影响。对于饲喂含低聚果糖日粮的仔猪,DGGE相似性分析表明,断奶后第一周粪便中的细菌群落发育迅速,之后相对缓慢。