Konstantinov Sergey R, Zhu Wei-Yun, Williams Barbara A, Tamminga Seerp, Vos Willem M, Akkermans Antoon D L
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, Netherlands.
FEMS Microbiol Ecol. 2003 Mar 1;43(2):225-35. doi: 10.1111/j.1574-6941.2003.tb01062.x.
The effect of fermentable carbohydrates (sugar beet pulp and fructooligosaccharides) on the faecal bacterial communities of weaning piglets was analysed using 16S rDNA-based approaches. Amplicons of the V6-V8 variable regions of bacterial 16S rDNA were analysed by denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Differences in piglet faecal bacterial community structure were determined based on the Dice coefficients for pairwise comparison of the DGGE fingerprints and revealed significant changes in the faecal microbiota immediately after weaning. Piglets fed with fermentable carbohydrates showed a higher bacterial diversity and a more rapid stabilisation of the bacterial community compared with that of the animals fed with the control diet. Thirteen dominant DGGE bands were matched with sequences that showed 91-97% similarity to those derived from the Clostridium coccoides group and the Clostridium leptum subgroup. Amplicons related to Ruminococcus-like species were found in all DGGE fingerprints derived from pigs on the diet containing sugar beet pulp and fructooligosaccharides, but not in pigs on the control diet. These results indicate that these bacteria may play a role in the utilisation of dietary fibres.
采用基于16S rDNA的方法分析了可发酵碳水化合物（甜菜粕和低聚果糖）对断奶仔猪粪便细菌群落的影响。通过变性梯度凝胶电泳（DGGE）、克隆和测序分析细菌16S rDNA V6 - V8可变区的扩增子。基于DGGE指纹图谱的成对比较的迪西系数确定仔猪粪便细菌群落结构的差异，并揭示断奶后粪便微生物群立即发生显著变化。与饲喂对照日粮的动物相比，饲喂可发酵碳水化合物的仔猪表现出更高的细菌多样性和更快速的细菌群落稳定。13条主要的DGGE条带与序列匹配，这些序列与源自梭状芽孢杆菌属球菌组和纤细梭菌亚组的序列具有91 - 97%的相似性。在所有来自饲喂含甜菜粕和低聚果糖日粮的猪的DGGE指纹图谱中均发现了与瘤胃球菌样物种相关的扩增子，但在饲喂对照日粮的猪中未发现。这些结果表明这些细菌可能在膳食纤维的利用中发挥作用。
