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[肺炎克雷伯菌属、肠杆菌属、沙雷菌属、大肠埃希菌属细菌将20种氨基酸作为唯一氮源和碳源的利用情况]

[Profiles of the utilization of 20 amino acids as the only source of nitrogen and carbon in bacteria of the genera Klebsiella, Enterobacter, Serratia, Escherichia].

作者信息

Sivolodskiĭ E P

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2005 Sep-Oct(5):11-4.

Abstract

The profiles of the utilization of 20 protein amino acids in 118 Klebsiella pneumoniae sub- sp. pneumoniae, K. oxytoca, K. planticola, K. mobilis, Enterobacter cloacae, Serratia marscescens, S. liquefaciens, Escherichia coli strains isolated from clinical material were studied. The utilization of amino acids was determined on minimal saline agar containing amino acid as the only source of nitrogen and carbon; the results were evaluated after 72-hour incubation at 37 degrees C. 17 profiles of amino-acid utilization were thus determined, most of them genus-specific in enterobacteria: Klebsiella (profiles No. 1--6, 9, 10), Enterobacter (No. 11--13), Serratia (No. 14--16), Escherichia (No. 17). The full coincidence of amino-acid utilization profiles in bacteria of K. mobilis (No. 1, 6) and K. pneumoniae subsp. pneumoniae with out of such profiles in bacteria of the genera Enterobacter, Serratia, Escherichia was established, which confirmed that K. mobilis (formerly Enterobacter aerogenes) belonged to the genus Klebsiella.

摘要

研究了从临床材料中分离出的118株肺炎克雷伯菌、产酸克雷伯菌、植生克雷伯菌、催娩克雷伯菌、阴沟肠杆菌、黏质沙雷菌、液化沙雷菌和大肠杆菌对20种蛋白质氨基酸的利用情况。氨基酸的利用是在以氨基酸作为唯一氮源和碳源的微量盐琼脂上测定的;在37℃孵育72小时后评估结果。由此确定了17种氨基酸利用模式,其中大多数在肠杆菌中具有属特异性:克雷伯菌属(模式编号1-6、9、10)、肠杆菌属(编号11-13)、沙雷菌属(编号14-16)、埃希菌属(编号17)。确定了催娩克雷伯菌(编号1、6)和肺炎克雷伯菌亚种细菌的氨基酸利用模式与肠杆菌属、沙雷菌属、埃希菌属细菌的此类模式完全一致,这证实了催娩克雷伯菌(以前称为产气肠杆菌)属于克雷伯菌属。

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