Rouf M A, Lomprey R F
J Bacteriol. 1968 Sep;96(3):617-22. doi: 10.1128/jb.96.3.617-622.1968.
We have isolated and identified nine cultures of aerobic bacteria capable of growing on an elective medium containing uric acid as the only source of carbon, nitrogen, and energy. Four of these cultures were identified as Aerobacter aerogenes, two as Klebsiella pneumoniae, and the remainder as Serratia killiensis, Pseudomonas aeruginosa, and Bacillus species. Another culture identified as P. fluorescens required both glucose and uric acid for growth. When 23 laboratory stock cultures were inoculated into the uric acid medium, A. aerogenes, B. subtilis, Mycobacterium phlei, P. aeruginosa, and S. marcescens were able to grow. These five cultures also grew when the uric acid was replaced with adenine, guanine, hypoxanthine, xanthine, or allantoin, but growth was poor. In all of these media, including the uric acid medium, addition of glucose along with the nitrogenous compounds yielded good growth. Induction experiments demonstrated that the ability of A. aerogenes, K. pneumoniae, P. aeruginosa, P. fluorescens, S. kiliensis, S. marcescens, B. subtilis, and Bacillus sp. to degrade uric acid is an induced property. Of these organisms, only Bacillus sp. accumulated a small amount of intracellular uric acid.
我们已经分离并鉴定出九种需氧细菌培养物，它们能够在以尿酸作为唯一碳源、氮源和能源的选择性培养基上生长。其中四种培养物被鉴定为产气气杆菌，两种为肺炎克雷伯菌，其余的为杀鲑气单胞菌、铜绿假单胞菌和芽孢杆菌属。另一种被鉴定为荧光假单胞菌的培养物生长需要葡萄糖和尿酸。当将23种实验室保藏培养物接种到尿酸培养基中时，产气气杆菌、枯草芽孢杆菌、草分枝杆菌、铜绿假单胞菌和黏质沙雷氏菌能够生长。当尿酸被腺嘌呤、鸟嘌呤、次黄嘌呤、黄嘌呤或尿囊素取代时，这五种培养物也能生长，但生长较差。在所有这些培养基中，包括尿酸培养基，添加葡萄糖以及含氮化合物会产生良好的生长效果。诱导实验表明，产气气杆菌、肺炎克雷伯菌、铜绿假单胞菌、荧光假单胞菌、杀鲑气单胞菌、黏质沙雷氏菌、枯草芽孢杆菌和芽孢杆菌属降解尿酸的能力是一种诱导特性。在这些生物体中，只有芽孢杆菌属积累了少量细胞内尿酸。
