Oka Kazue, Kimura Tomoko, Otsuka Masato, Ohmori Shinji
Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 2;830(1):173-7. doi: 10.1016/j.jchromb.2005.10.033. Epub 2005 Nov 8.
Threonine was oxidized into acetaldehyde at 0 degrees C for 30 min with periodic acid. The acetaldehyde formed was converted to a hydrazone with 2,4-dinitrophenyhydrazine. The hydrazone was extracted with n-heptane and quantified by gas liquid chromatography with electron capture detection. An internal standard, 2-amino-3-hydroxyhexanoic acid, was used. The calibration curve of threonine was linear up to 200 nmol in 200 microl sample solution and the determination limit of threonine was 1 nmol in 200 microl sample solution. The recoveries were 100.0, 94.0 and 100.0% from homogenates of octopus tentacles and blood plasma and rat livers, respectively. This method was applied to the determination of threonine in tissues of rats given threonine and starved octopuses. This threonine determination method has been used for studies on the metabolism of d-lactate.
苏氨酸在0℃下用高碘酸氧化30分钟生成乙醛。生成的乙醛与2,4-二硝基苯肼反应生成腙。用正庚烷萃取腙，并通过带有电子捕获检测的气相色谱法定量。使用内标2-氨基-3-羟基己酸。在200微升样品溶液中，苏氨酸的校准曲线在高达200纳摩尔时呈线性，在200微升样品溶液中苏氨酸的测定限为1纳摩尔。从章鱼触手、血浆和大鼠肝脏匀浆中的回收率分别为100.0%、94.0%和100.0%。该方法应用于测定给予苏氨酸的大鼠和饥饿章鱼组织中的苏氨酸。这种苏氨酸测定方法已用于d-乳酸代谢的研究。
