Švec Pavel, Vancanneyt Marc, Koort Joanna, Naser Sabri M, Hoste Bart, Vihavainen Elina, Vandamme Peter, Swings Jean, Björkroth Johanna
Czech Collection of Microorganisms, Faculty of Science, Masaryk University Brno, Tvrdého 14, 602 00 Brno, Czech Republic.
BCCM/LMG Bacteria Collection, Faculty of Sciences, Ghent University, Ledeganckstraat 35, B 9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2479-2484. doi: 10.1099/ijs.0.63851-0.
The taxonomic position of two bovine strains, LMG 13603 and LMG 14595, assigned to the species Enterococcus raffinosus on the basis of biochemical features, was reinvestigated. Both reference strains and two other isolates, 6/1 (=LMG 22829) originating from a charcoal-broiled river lamprey and IE38.4 (=LMG 22830) from the air of a poultry slaughter by-product processing plant, occupied a clearly separate position, on the basis of sequence analysis of the housekeeping gene pheS (encoding the phenylalanyl-tRNA synthase alpha-subunit), relative to the type strain of E. raffinosus and all other enterococcal species with validly published names. 16S rRNA gene sequencing of strains LMG 13603, LMG 14595, 6/1 and IE38.4 confirmed their phylogenetic position in the Enterococcus avium species group, there being more than 99 % 16S rRNA gene sequence similarity to most members of the group, including E. raffinosus, and revealed Enterococcus pseudoavium as the closest phylogenetic relative (99.8-99.9 %). Further phenotypic and genotypic analyses using whole-cell-protein electrophoresis, (GTG)(5)-PCR fingerprinting, ribotyping and DNA-DNA hybridization experiments demonstrated that all four strains represent a novel enterococcal species, for which the name Enterococcus devriesei sp. nov. is proposed. The type strain is LMG 14595T (=CCM 7299T).
对两株牛源菌株LMG 13603和LMG 14595的分类地位进行了重新研究，这两株菌基于生化特征被归为棉籽糖肠球菌。参考菌株以及另外两株分离株，即源自炭烤河鳗的6/1（=LMG 22829）和源自家禽屠宰副产品加工厂空气的IE38.4（=LMG 22830），根据管家基因pheS（编码苯丙氨酰 - tRNA合成酶α亚基）的序列分析，相对于棉籽糖肠球菌模式菌株及所有其他有效发表名称的肠球菌属物种，占据明显不同的位置。对菌株LMG 13603、LMG 14595、6/1和IE38.4进行16S rRNA基因测序，证实了它们在鸟肠球菌物种组中的系统发育位置，与该组的大多数成员（包括棉籽糖肠球菌）有超过99%的16S rRNA基因序列相似性，并揭示伪鸟肠球菌是最近的系统发育亲缘种（99.8 - 99.9%）。使用全细胞蛋白电泳、(GTG)(5)-PCR指纹图谱、核糖体分型和DNA - DNA杂交实验进行的进一步表型和基因型分析表明，所有四株菌株代表一个新的肠球菌物种，为此提出名为德弗里斯肠球菌（Enterococcus devriesei sp. nov.）。模式菌株为LMG 14595T（=CCM 7299T）。
