基质金属蛋白酶抑制剂RECK基因在前列腺细胞株中的表达及意义
[Expression and significance of the matrix metalloproteinase inhibitor RECK gene in prostate cell strains].
作者信息
Xu Zhen-yu, Gao Jian-ping, Zhang Zheng-pu, Ge Jing-ping, Xu Chuan-liang, Sun Ying-hao
机构信息
Department of Urology, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
出版信息
Zhonghua Nan Ke Xue. 2005 Oct;11(10):727-30.
OBJECTIVE
To investigate the expression and significance of RECK gene and MMP-9 in prostate cell strains such as BPH-1, DU45, LNCaP and PC-3.
METHODS
The expression of mRNA of RECK and MMP-9 was measured by RT-PCR and RECK protein was evaluated by Western blot.
RESULTS
The mRNA level of the RECK gene in the prostate carcinoma cell strains, such as DU45, LNCaP and PC-3, was lower than that in the benign prostate hyperplasia cell line BPH-1, while MMP-9 had a higher expression. The protein level of RECK in DU-45, LNCaP and PC-3 was lower than that in the BPH-1.
CONCLUSION
The RECK gene is supposed to be a kind of tumor suppressor gene, which may act by inhibiting the activity of MMP-9.
目的
探讨RECK基因和基质金属蛋白酶-9（MMP-9）在前列腺细胞株如BPH-1、DU45、LNCaP和PC-3中的表达及意义。
方法
采用逆转录聚合酶链反应（RT-PCR）检测RECK和MMP-9的mRNA表达水平，并用蛋白质印迹法评估RECK蛋白表达情况。
结果
DU45、LNCaP和PC-3等前列腺癌细胞株中RECK基因的mRNA水平低于良性前列腺增生细胞系BPH-1，而MMP-9表达较高。DU-45、LNCaP和PC-3中RECK蛋白水平低于BPH-1。
结论
RECK基因可能是一种肿瘤抑制基因，其可能通过抑制MMP-9的活性发挥作用。
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