[具有良好絮凝性的优良面包酵母菌株的选育]

[Breeding of excellent baker's yeast strain with good flocculation].

作者信息

Liu Chunxiu, He Xiuping, Jiang Sixin, Qu Na, Zhang Borun

机构信息

Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China.

出版信息

Wei Sheng Wu Xue Bao. 2003 Oct;43(5):659-65.

PMID:16281566

Abstract

An excellent baker's yeast strain ZLTH-58 (MATa/alpha, leu-, FLO1) with high biomass, high-sugar-tolerance and good flocculation was constructed by primary screening, isolation of haploid, mutagenesis, cloning and expression of FLO1 gene and hybridization. The results showed that strain ZLTH-58 had excellent properties of the parental strains BL56 and BL61. The biomass of strain ZLTH-58 was 1.21 times of the parental strain BL56; The ability of high-sugar-tolerance of strain ZLTH-58 was higher than the parental strain BL61. The ability of flocculation of strain ZLTH-58 was better than the parental strains BL56 and BL61. The factors that affected the biomass of strain ZLTH-58 were also detected. Biomass reached 83.06 g/L under the optimal fermentation conditions, having a 1.35-fold improvement. Strain ZLTH-58 is also stable in genetics characters by analysis of genetic stability and it has the potential for use in industrial processes.

摘要

通过初筛、单倍体分离、诱变、FLO1基因克隆与表达及杂交构建了一株优良的面包酵母菌株ZLTH-58（MATa/alpha，亮氨酸缺陷型，FLO1），该菌株具有高生物量、高耐糖性和良好的絮凝性。结果表明，菌株ZLTH-58具有亲本菌株BL56和BL61的优良特性。菌株ZLTH-58的生物量是亲本菌株BL56的1.21倍；菌株ZLTH-58的高耐糖能力高于亲本菌株BL61。菌株ZLTH-58的絮凝能力优于亲本菌株BL56和BL61。还检测了影响菌株ZLTH-58生物量的因素。在最佳发酵条件下，生物量达到83.06 g/L，提高了1.35倍。通过遗传稳定性分析，菌株ZLTH-58在遗传特性上也很稳定，具有工业应用潜力。