Kumar Swati, Kohlhoff Stephan A, Gelling Maureen, Roblin Patricia M, Kutlin Andrei, Kahane Simona, Friedman Maureen G, Hammerschlag Margaret R
Division of Infectious Diseases, Department of Pediatrics, State University of New York Downstate Medical Center, Brooklyn, NY, USA.
Pediatr Infect Dis J. 2005 Nov;24(11):989-92. doi: 10.1097/01.inf.0000183755.24578.0b.
Simkania negevensis is a Chlamydia-like intracellular organism that is prevalent in populations from a wide range of geographic areas. The role of the organism in respiratory disease in the United States is unknown.
To study the association between infection with S. negevensis and bronchiolitis, pneumonia or asthma in Brooklyn, New York.
Pediatric and adult inpatients/outpatients with bronchiolitis, pneumonia or asthma were recruited, and a similar number of healthy control subjects were enrolled. Nasopharyngeal swabs were obtained for culture of S. negevensis and Chlamydia pneumoniae and polymerase chain reaction detection of S. negevensis. Sera were obtained for measurement of antibodies to S. negevensis and C. pneumoniae.
One hundred eighty-eight patients and 110 healthy control subjects were enrolled. S. negevensis serologic assays were positive for 18% of patients, compared with 29% of control subjects (P = 0.09). S. negevensis DNA was detected by PCR for 17% of case subjects and 23% of control subjects (P = 0.25). S. negevensis was isolated by culture for 1 patient with bronchiolitis. C. pneumoniae IgG and S. negevensis IgG were found to increase with increasing age, ie, 14%, 50% and 78% (C. pneumoniae) and 13%, 17% and 33% (S. negevensis) for subjects 0-18 months, 18 months-18 years and older than 18 years of age, respectively.
S. negevensis was not a significant respiratory pathogen in Brooklyn, NY, during the period of the study.
内基西氏西曼卡菌是一种类似衣原体的细胞内生物体，在广泛地理区域的人群中普遍存在。该生物体在美国呼吸道疾病中的作用尚不清楚。
研究纽约布鲁克林内基西氏西曼卡菌感染与细支气管炎、肺炎或哮喘之间的关联。
招募患有细支气管炎、肺炎或哮喘的儿科和成人住院/门诊患者，并纳入数量相近的健康对照受试者。采集鼻咽拭子用于内基西氏西曼卡菌和肺炎衣原体培养以及内基西氏西曼卡菌的聚合酶链反应检测。采集血清用于检测内基西氏西曼卡菌和肺炎衣原体抗体。
共招募了188例患者和110名健康对照受试者。18%的患者内基西氏西曼卡菌血清学检测呈阳性，而对照受试者为29%（P = 0.09）。通过聚合酶链反应检测到17%的病例受试者和23%的对照受试者存在内基西氏西曼卡菌DNA（P = 0.25）。通过培养从1例细支气管炎患者中分离出内基西氏西曼卡菌。发现肺炎衣原体IgG和内基西氏西曼卡菌IgG随年龄增长而增加，即0至18个月、18个月至18岁和18岁以上受试者中，肺炎衣原体IgG阳性率分别为14%、50%和78%，内基西氏西曼卡菌IgG阳性率分别为13%、17%和33%。
在研究期间，内基西氏西曼卡菌不是纽约布鲁克林地区重要的呼吸道病原体。
