Portnoy Victoria, Evguenieva-Hackenberg Elena, Klein Franziska, Walter Pamela, Lorentzen Esben, Klug Gabriele, Schuster Gadi
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel.
EMBO Rep. 2005 Dec;6(12):1188-93. doi: 10.1038/sj.embor.7400571.
The addition of poly(A) tails to RNA is a phenomenon common to all organisms examined so far. No homologues of the known polyadenylating enzymes are found in Archaea and little is known concerning the mechanisms of messenger RNA degradation in these organisms. Hyperthermophiles of the genus Sulfolobus contain a protein complex with high similarity to the exosome, which is known to degrade RNA in eukaryotes. Halophilic Archaea, however, do not encode homologues of these eukaryotic exosome components. In this work, we analysed RNA polyadenylation and degradation in the archaea Sulfolobus solfataricus and Haloferax volcanii. No RNA polyadenylation was detected in the halophilic archaeon H. volcanii. However, RNA polynucleotidylation occurred in hyperthermophiles of the genus Sulfolobus and was mediated by the archaea exosome complex. Together, our results identify the first organism without RNA polyadenylation and show a polyadenylation activity of the archaea exosome.
给RNA添加聚腺苷酸尾巴是迄今为止所有已检测生物中都存在的一种现象。在古菌中未发现已知聚腺苷酸化酶的同源物，并且对于这些生物中信使RNA的降解机制知之甚少。硫磺菌属的嗜热菌含有一种与外切体高度相似的蛋白质复合物，已知该复合物在真核生物中可降解RNA。然而，嗜盐古菌并不编码这些真核外切体成分的同源物。在这项研究中，我们分析了古菌嗜热栖热菌和沃氏嗜盐菌中的RNA聚腺苷酸化和降解情况。在嗜盐古菌沃氏嗜盐菌中未检测到RNA聚腺苷酸化。然而，在硫磺菌属的嗜热菌中发生了RNA多聚核苷酸化，并且由古菌外切体复合物介导。总之，我们的结果鉴定出了第一种没有RNA聚腺苷酸化的生物，并展示了古菌外切体的聚腺苷酸化活性。
