冈田酸处理的HeLa细胞中“持久性”核仁内染色质的定位

Localization of chromatin in "persistent" nucleoli in okadaic acid treated HeLa cells.

作者信息

Ghosh S, Paweletz N

机构信息

Centre of Advanced Study in Botany, University of Calcutta, India.

出版信息

Cell Biol Int Rep. 1992 May;16(5):475-80. doi: 10.1016/s0309-1651(06)80066-4.

DOI:10.1016/s0309-1651(06)80066-4

PMID:1628320

Abstract

In okadaic acid treated HeLa cells, the chromosomes sometimes condense without being accompanied by nuclear envelope breakdown. These cells show "persistent" nucleoli. Within these "persistent" nucleoli the intranucleolar chromatin condenses and can be observed in the region of the dense nucleolar component (DNC) of the nucleoli. Other nucleolar components, namely the fibrillar centre (FC) and the granular component (GC) remain unchanged. These observations strongly speak for the localization of nucleolar chromatin (ribosomal cistrons) within the dense nucleolar component of the interphase nucleolus.

摘要

在经冈田酸处理的HeLa细胞中，染色体有时会凝聚，却不伴有核膜破裂。这些细胞呈现出“持续存在”的核仁。在这些“持续存在”的核仁内，核仁内染色质凝聚，并且可以在核仁的致密核仁成分（DNC）区域观察到。其他核仁成分，即纤维中心（FC）和颗粒成分（GC）则保持不变。这些观察结果有力地证明了核仁染色质（核糖体顺反子）定位于间期核仁的致密核仁成分内。