Pan Chun-ling, Pan Ya-ping, Lin Li, Zhao Jian, Zhang Dong-mei
Dept. of Periodontology, School of Stomatology, China Medical University, Shenyang 110002, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2005 Oct;23(5):377-9.
To detect Porphyromonas gingivalis (P. gingivalis) in buccal epithelial cells and subgingival plaque from periodontally healthy subjects and patients with chronic periodontitis.
40 subjects were included in the healthy group and 39 subjects were included in the diseased group in this study. Cells and subgingival plaque samples were collected. The extracted DNA was amplified with universal primers and P. gingivalis species-specific primer.
P. gingivalis was detected in 37.5% of subgingival plaque samples and 32.5% of buccal mucosa samples in the healthy subjects, but 69.23% of subgingival plaque samples and 46.15% of buccal mucosa samples in the periodontitis group. Highly statistically significant differences were observed between healthy and periodontitis groups in the detections of P. gingivalis of subgingival plaque samples.
P. gingivalis may be one of oral flora because it can be detected in the healthy population and not lead to destruction of supporting structures of the teeth.
检测牙周健康受试者和慢性牙周炎患者颊上皮细胞及龈下菌斑中的牙龈卟啉单胞菌（P. gingivalis）。
本研究中，健康组纳入40名受试者，疾病组纳入39名受试者。采集细胞和龈下菌斑样本。提取的DNA用通用引物和牙龈卟啉单胞菌特异性引物进行扩增。
健康受试者中，37.5%的龈下菌斑样本和32.5%的颊黏膜样本检测到牙龈卟啉单胞菌，而牙周炎组中，69.23%的龈下菌斑样本和46.15%的颊黏膜样本检测到该菌。健康组和牙周炎组在龈下菌斑样本牙龈卟啉单胞菌检测方面存在高度统计学显著差异。
牙龈卟啉单胞菌可能是口腔菌群之一，因为在健康人群中可检测到且不会导致牙齿支持结构破坏。
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