School of Dental Sciences, Newcastle University, Newcastle, UK.
J Periodontal Res. 2012 Aug;47(4):470-8. doi: 10.1111/j.1600-0765.2011.01455.x. Epub 2012 Jan 3.
Diabetes is a recognized risk factor for periodontitis. There are conflicting data regarding whether healthy diabetic patients or diabetic patients with chronic periodontitis have an altered subgingival microbiota compared with nondiabetic individuals. The aim of the present study was to detect quantitative differences in selected periodontopathogens in the subgingival plaque of diabetic patients using TaqMan quantitative PCR.
Type 2 diabetes mellitus patients with (n=9) or without chronic periodontal disease (n=15) were recruited and matched to nondiabetic control subjects (n=12 periodontally healthy, n=12 chronic periodontitis). Subgingival plaque samples were collected from deep (>4 mm probing depth) and shallow sites (≤3 mm probing depth) using paper points, and Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Porphyromonas gingivalis were quantified.
Forty-eight subjects (69 samples) were recruited. Marked differences were seen in the levels of all three bacterial species, relative to the total bacterial population, according to periodontal health status. Using real-time quantitative PCR, bacterial counts for P. gingivalis were significantly higher in deep pockets of diabetic and nondiabetic subjects compared with periodontally healthy subjects (p<0.05) but did not differ significantly between diabetics and nondiabetics. A. actinomycetemcomitans was detected in all groups in low quantities, and counts did not differ significantly between groups (p>0.05). F. nucleatum was abundant in all groups, with no clear significant differences between groups. P. gingivalis was found in higher quantities in periodontitis than in periodontally healthy subjects (p<0.05). Statistically significant positive correlations were identified between pocket depth and counts for all three species tested (p<0.05).
A. actinomycetemcomitans, F. nucleatum and P. gingivalis were present in significantly different quantities and proportions in subgingival plaque, according to periodontal disease status. No significant differences were identified between the subgingival microbiota of type 2 diabetes mellitus patients compared with nondiabetic subjects.
糖尿病是牙周炎的公认危险因素。关于健康的糖尿病患者或患有慢性牙周炎的糖尿病患者与非糖尿病个体相比，其龈下微生物群是否存在改变，目前存在相互矛盾的数据。本研究旨在使用 TaqMan 定量 PCR 检测糖尿病患者龈下菌斑中选定的牙周病原体的定量差异。
招募了 9 例 2 型糖尿病伴慢性牙周病（CP）患者和 15 例不伴慢性牙周病（CP）的患者，并与 12 例牙周健康对照者（CPH）和 12 例慢性牙周炎对照者（CP）相匹配。使用牙间拭子从深（>4mm 探诊深度）和浅（≤3mm 探诊深度）部位采集龈下菌斑样本，并定量检测伴放线放线杆菌、核梭杆菌和牙龈卟啉单胞菌。
共招募了 48 名受试者（69 个样本）。根据牙周健康状况，三种细菌的水平与总细菌群相比存在明显差异。使用实时定量 PCR，与牙周健康的受试者相比，糖尿病和非糖尿病受试者的深袋中牙龈卟啉单胞菌的细菌计数明显更高（p<0.05），但糖尿病和非糖尿病患者之间无显著差异。所有组中均检测到少量的伴放线放线杆菌，组间细菌计数无显著差异（p>0.05）。核梭杆菌在所有组中均大量存在，组间无明显差异。牙龈卟啉单胞菌在牙周炎组中的数量高于牙周健康组（p<0.05）。所有检测的三种细菌的菌斑深度与细菌计数之间均存在显著正相关（p<0.05）。
根据牙周病状况，龈下菌斑中伴放线放线杆菌、核梭杆菌和牙龈卟啉单胞菌的数量和比例存在显著差异。2 型糖尿病患者的龈下微生物群与非糖尿病患者相比无明显差异。
