Limbeck Andreas, Handler Markus, Neuberger Bernhard, Klatzer Barbara, Puxbaum Hans
Institute of Chemical Technologies and Analytics, Vienna University of Technology, Austria.
Anal Chem. 2005 Nov 15;77(22):7288-93. doi: 10.1021/ac050953l.
A new approach for the carbon-specific determination of humic-like substances (HULIS) in atmospheric aerosols is presented. The method is based on a two-step isolation procedure of HULIS and the determination of HULIS carbon with a dissolved organic carbon analyzer. In the first step, a C18 solid-phase extraction is performed to separate HULIS from inorganic and hydrophilic organic sample constituents in aqueous sample solutions. The second isolation step is conducted on a strong anion exchanger to separate HULIS from remaining carbonaceous compounds. This ion chromatographic separation step including the subsequent on-line detection of HULIS carbon was performed fully automated to avoid the risk of sample contamination and to enhance the reproducibility of the method. With a 5-mL sample volume, a limit of detection of 1.0 mg C/L was obtained; this corresponds to an absolute amount of 5 microg of HULIS carbon. The reproducibility of the method given as the relative standard deviation was 4.3% (n = 10). The method was applied for the determination of water-soluble HULIS in airborne particulate matter. PM10 concentrations at an urban site in Vienna, Austria, ranged from around 0.1 to 1.8 microg of C/m(3) (n = 49); the fraction of water-soluble HULIS in OC was 12.1 +/- 7.2% (n = 49).
本文提出了一种测定大气气溶胶中类腐殖质（HULIS）碳含量的新方法。该方法基于两步分离程序来分离HULIS，并使用溶解有机碳分析仪测定HULIS碳含量。第一步，采用C18固相萃取法从水样中的无机和亲水性有机样品成分中分离出HULIS。第二步分离是在强阴离子交换剂上进行，以从残留的含碳化合物中分离出HULIS。包括后续在线检测HULIS碳的离子色谱分离步骤是完全自动化的，以避免样品污染风险并提高方法的重现性。对于5 mL的样品体积，检测限为1.0 mg C/L；这相当于5 μg的HULIS碳绝对量。以相对标准偏差表示的方法重现性为4.3%（n = 10）。该方法用于测定空气中颗粒物中水溶性HULIS。奥地利维也纳一个城市站点的PM10浓度范围约为0.1至1.8 μg C/m³（n = 49）；水溶性HULIS在有机碳中的占比为12.1±7.2%（n = 49）。
