Lucena Rafael, Cárdenas Soledad, Gallego Mercedes, Valcárcel Miguel
Department of Analytical Chemistry, Marie Curie Building (Annex), Campus de Rabanales, University of Córdoba, Spain.
Anal Chem. 2005 Nov 15;77(22):7472-7. doi: 10.1021/ac0507643.
A novel straightforward membrane-based sensor, which uses attenuated total reflection Fourier transform infrared (ATR-FT-IR) spectroscopy has been developed. The flow cell designed permits the on-line microliquid-liquid extraction of the target analyte into a organic solvent layer (OSL), which was deposited on the ATR surface using a sequential injection manifold. The aqueous and organic phases are separated via a commercial hydrophobic membrane placed on the PTFE piece of the cell. The main advantage of the proposed device is that the OSL can be created and regenerated in a continuous manner using the automatic manifold without opening the cell. The analytes are enriched into the OSL after diffusion through the membrane, which excludes the typical absorption bands of water. In addition, the behavior of different organic solvents was evaluated in order to increase the applicability and versatility of the proposed system. Finally, the analytical performance of the design was established for the detection and quantitation of Triton X100 in water.
一种新型的基于膜的直接传感器已被开发出来，它采用衰减全反射傅里叶变换红外（ATR-FT-IR）光谱技术。所设计的流通池允许将目标分析物在线微液-液萃取到有机溶剂层（OSL）中，该有机溶剂层使用顺序注射歧管沉积在ATR表面。水相和有机相通过放置在流通池PTFE部件上的商用疏水膜分离。该提议装置的主要优点是，使用自动歧管可以连续地创建和再生OSL，而无需打开流通池。分析物通过膜扩散后富集到OSL中，这排除了水的典型吸收带。此外，还评估了不同有机溶剂的性能，以提高所提议系统的适用性和通用性。最后，确定了该设计用于检测和定量水中曲拉通X100的分析性能。
