TaqMan逆转录聚合酶链反应及VERSANT HIV-1 RNA 3.0（分支DNA）检测法：母乳中HIV-1 RNA病毒载量的定量分析
TaqMan RT-PCR and VERSANT HIV-1 RNA 3.0 (bDNA) assay Quantification of HIV-1 RNA viral load in breast milk.
作者信息
Israel-Ballard Kiersten, Ziermann Rainer, Leutenegger Christian, Di Canzio James, Leung Kimmy, Strom Lynn, Abrams Barbara, Chantry Caroline
机构信息
Department of Epidemiology, School of Public Health, University of California, 140 Earl Warren Hall, Berkeley, CA 94720-7360, USA.
出版信息
J Clin Virol. 2005 Dec;34(4):253-6. doi: 10.1016/j.jcv.2005.02.013.
BACKGROUND
Transmission of HIV via breast milk is a primary cause of pediatric HIV infection in developing countries. Reliable methods to detect breast milk viral load are important.
OBJECTIVE
To correlate the ability of the VERSANT HIV 3.0 (bDNA) assay to real-time (RT) TaqMan PCR in quantifying breast milk HIV-1 RNA.
STUDY DESIGN
Forty-six breast milk samples that had been spiked with cell-free HIV-1 and eight samples spiked with cell-associated HIV-1 were assayed for HIV-1 RNA by both VERSANT HIV 3.0 and TaqMan RNA assays.
RESULTS
Only assays on the cell-free samples were statistically compared. Both a Deming regression slope and a Bland-Altman slope indicated a linear relationship between the two assays. TaqMan quantitations were on average 2.6 times higher than those of HIV 3.0. A linear relationship was observed between serial dilutions of spiked cell-free HIV-1 and both the VERSANT HIV 3.0 and the TaqMan RNA assays.
CONCLUSION
The two methods correlated well although the VERSANT HIV 3.0 research protocol quantified HIV-1 RNA slightly lower than TaqMan.
背景
在发展中国家，通过母乳传播艾滋病毒是儿童感染艾滋病毒的主要原因。检测母乳病毒载量的可靠方法很重要。
目的
比较VERSANT HIV 3.0（分支DNA）检测法与实时（RT）TaqMan聚合酶链反应在定量母乳中HIV-1 RNA方面的能力。
研究设计
对46份添加了游离HIV-1的母乳样本和8份添加了细胞相关HIV-1的样本，采用VERSANT HIV 3.0检测法和TaqMan RNA检测法检测HIV-1 RNA。
结果
仅对游离样本的检测结果进行了统计学比较。戴明回归斜率和布兰德-奥特曼斜率均表明两种检测方法之间存在线性关系。TaqMan定量结果平均比HIV 3.0高2.6倍。在添加的游离HIV-1系列稀释液与VERSANT HIV 3.0检测法和TaqMan RNA检测法之间均观察到线性关系。
结论
两种方法相关性良好，尽管VERSANT HIV 3.0研究方案对HIV-1 RNA的定量略低于TaqMan。
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