Expression pattern of matrix metalloproteinases-13 in a rat model of alcoholic liver fibrosis.

BACKGROUND

Interstitial collagenase has been considered as an essential enzyme for collagenolysis in liver fibrosis, because type I and III collagens increase predominantly in liver fibrosis. The present study aimed to demonstrate the gene expression of matrix metalloproteinases-13 (MMP-13) in the progressive phases of ethanol induced experimental liver fibrosis in rats.

METHODS

Thirty-four Sprague-Dawley rats were randomly divided into two groups. The experimental group (24 rats) was given ethanol (44%, 7 g/kg) every day and the control group (10) was given normal saline. Liver samples were harvested from experimental rats at 4, 12 and 24 weeks respectively. The kinetics of MMP-13 mRNA expression was assayed by semi-quantity reverse transcriptase-polymerase chain reaction (RT-PCR).

RESULTS

In normal rat liver, a faint band for MMP-13 mRNA was observed by RT-PCR (0.24+/-0.41). The gene expression of MMP-13 was increased in the liver of the rats treated with ethanol for 4 weeks (0.62+/-0.54), but it was not considered statistically significant (P>0.05). And the livers from 12-week-treated rats showed a marked mRNA expression (1.65+/-0.47, P<0.01). Once fibrosis became prominent (24 weeks), a faint band of MMP-13 mRNA was observed (0.39+/-0.25).

CONCLUSION

MMP-13 participates in the degradation of newly-formed matrix in the early phase of rat liver fibrosis induced by ethanol, and it was induced in a distinct time frame.