Liu Xiu-ling, Zhnag Hong, Wang Fei
Department of Pharmacy, Renmin Hospital, Wuhan University, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jul;31(13):1097-100.
To investigate the effects of Hovenia dulcis extract on mRNA expression of MMP-13 and TIMP-1 mRNA in hepatic tissue in experimental rats.
48 male Sprague-Dawley rats were randomly divided into 2 groups: normal group (16) and model group (32), hepatic fibrosis was induced by CCl4 for 6 weeks in rats, 8 rats were sacrificed at the end of the 6th week from every group respectively, HE staining of hepatic tissue was performed; In the model group, rats randomly subdivided into 3 groups: spontaneous recovery group, control group and medication administration group, 8 rats were sacrificed at the end of the 12th week from every group respectively, the mRNA levels of MMP-13 and TIMP-1 in hepatic tissue were assayed by semi-quantitative RT-PCR.
The mRNA expression of MMP-13 among the 4 groups were not statistically significant, but the mRNA expression of TIMP-1 among the 4 groups were statiscally significant. The levels of TIMP-1 mRNA were significantly increased in control group and medication administration group compared, with those in the model group (P < 0.05), and reverse effects of medication administration groups were significantly high than those of control group (P < 0.05).
Inhibition of the mRNA expression of TIMP-1 may be the mechanism of reversing hepatic fibrosis H. dulcis, for thus collogen degradation system was recoveried gradually.
探讨枳椇子提取物对实验性大鼠肝组织中基质金属蛋白酶-13（MMP-13）和基质金属蛋白酶组织抑制因子-1（TIMP-1）mRNA表达的影响。
48只雄性Sprague-Dawley大鼠随机分为2组：正常组（16只）和模型组（32只），采用四氯化碳诱导大鼠肝纤维化6周，每组分别在第6周结束时处死8只大鼠，进行肝组织苏木精-伊红（HE）染色；在模型组中，大鼠随机再分为3组：自然恢复组、对照组和给药组，每组分别在第12周结束时处死8只大鼠，采用半定量逆转录聚合酶链反应（RT-PCR）检测肝组织中MMP-13和TIMP-1的mRNA水平。
4组中MMP-13的mRNA表达无统计学意义，但4组中TIMP-1的mRNA表达有统计学意义。与模型组比较，对照组和给药组TIMP-1 mRNA水平显著升高（P<0.05），且给药组的逆转作用显著高于对照组（P<0.05）。
抑制TIMP-1的mRNA表达可能是枳椇子逆转肝纤维化的机制，从而使胶原降解系统逐渐恢复。
